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A multidimensional cell wall analysis was performed to generate a reference profile for leaf and stem biomass from several miscanthus genotypes harvested at three developmentally distinct time points. A comprehensive suite of 155 monoclonal antibodies was used to monitor changes in distribution, structure and extractability of noncellulosic cell wall matrix glycans.
Glycan microarrays complemented with immunohistochemistry elucidated the nature of compositional variation, and in situ distribution of carbohydrate epitopes. Key observations demonstrated that there are crucial differences in miscanthus cell wall glycomes, which may impact biomass amenability to deconstruction.
For the first time, variations in miscanthus cell wall glycan components were comprehensively characterized across different harvests, organs and genotypes, to generate a representative reference profile for miscanthus cell wall biomass. Ultimately, this portrait of the miscanthus cell wall will help to steer breeding and genetic engineering strategies for the development of superior energy crops.
- Cell Wall
- cell wall
- Crops, Agricultural/growth & development
- Plant Leaves/metabolism
- Poaceae/growth & development
- Principal Component Analysis
- Plant Development
- Plant Stems/metabolism
- Cell Wall/metabolism
FingerprintDive into the research topics of 'A cell wall reference profile for Miscanthus bioenergy crops highlights compositional and structural variations associated with development and organ origin'. Together they form a unique fingerprint.
- Faculty of Earth and Life Sciences, Institute of Biological, Environmental & Rural Sciences (IBERS) - Reader
Person: Teaching And Research
- 1 Finished
01 Apr 2012 → 31 Mar 2017
Project: Externally funded research