A comparison of two approaches to extracting Cryptosporidium DNA from human stools as measured by a real-time PCR assay

K. Elwin, G. Robinson, S. J. Hadfield, H. V. Fairclough, M. Iturriza-Gomara, R. M. Chalmers

Research output: Contribution to journalArticlepeer-review

31 Citations (SciVal)

Abstract

Direct extraction of Cryptosporidium DNA from 46 stools by bead-beating, guanidine thiocyanate and silica purification provided slightly lower PCR positivity (93.5% vs. 100%) and higher threshold cycle values (mean 34.93 vs. 28.03; P = 0.00) than spin-column extraction from boiled, semi-purified oocyst suspensions. However, direct extraction is cheaper, and amenable to automation.
Original languageEnglish
Pages (from-to)38-40
Number of pages3
JournalJournal of Microbiological Methods
Volume89
Issue number1
DOIs
Publication statusPublished - 01 Apr 2012

Keywords

  • Cryptosporidium
  • DNA extraction
  • Real time PCR

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