TY - JOUR
T1 - A defined antigen skin test for the diagnosis of bovine tuberculosis
AU - Srinivasan, Sreenidhi
AU - Jones, Gareth
AU - Veerasami, Maroudam
AU - Steinbach, Sabine
AU - Holder, Thomas
AU - Zewude, Aboma
AU - Fromsa, Abebe
AU - Ameni, Gobena
AU - Easterling, Laurel
AU - Bakker, Douwe
AU - Jeuleff, Nicholas
AU - Gifford, Glen
AU - Hewinson, Glyn
AU - Vordermeier, Hans-Martin
AU - Kapur, Vivek
N1 - Funding Information:
We acknowledge the excellent technical support provided by veterinary staff at the APHA, UK, and the Addis Ababa University, Ethiopia. This study was supported by a grant (OPP1176950) from the Bill and Melinda Gates Foundation and the U.K. Department for International Development. We also acknowledge the Department for Environment, Food and Rural Affairs (DEFRA), UK, for the use of cattle infected under project SE3304. R.G.H. and H.M.V. are supported by the Welsh Government and the European Regional Development Fund Ser Cymru II project 80761-AU185. V.K. serves as a senior advisor and paid consultant to the Bill and Melinda Gates Foundation, a funding agency for this study. However, the funding agency did not have any role in the design, conduct, analysis, interpretation, or writing of this study.
Publisher Copyright:
Copyright © 2019 The Authors,
PY - 2019/7/17
Y1 - 2019/7/17
N2 - Bovine tuberculosis (bTB) is a major zoonotic disease of cattle that is endemic in much of the world, limiting livestock productivity and representing a global public health threat. Because the standard tuberculin skin test precludes implementation of Bacille Calmette-Guérin (BCG) vaccine–based control programs, we here developed and evaluated a novel peptide-based defined antigen skin test (DST) to diagnose bTB and to differentiate infected from vaccinated animals (DIVA). The results, in laboratory assays and in experimentally or naturally infected animals, demonstrate that the peptide-based DST provides DIVA capability and equal or superior performance over the extant standard tuberculin surveillance test. Together with the ease of chemical synthesis, quality control, and lower burden for regulatory approval compared with recombinant antigens, the results of our studies show that the DST considerably improves a century-old standard and enables the development and implementation of critically needed surveillance and vaccination programs to accelerate bTB control.
AB - Bovine tuberculosis (bTB) is a major zoonotic disease of cattle that is endemic in much of the world, limiting livestock productivity and representing a global public health threat. Because the standard tuberculin skin test precludes implementation of Bacille Calmette-Guérin (BCG) vaccine–based control programs, we here developed and evaluated a novel peptide-based defined antigen skin test (DST) to diagnose bTB and to differentiate infected from vaccinated animals (DIVA). The results, in laboratory assays and in experimentally or naturally infected animals, demonstrate that the peptide-based DST provides DIVA capability and equal or superior performance over the extant standard tuberculin surveillance test. Together with the ease of chemical synthesis, quality control, and lower burden for regulatory approval compared with recombinant antigens, the results of our studies show that the DST considerably improves a century-old standard and enables the development and implementation of critically needed surveillance and vaccination programs to accelerate bTB control.
KW - Animals
KW - Antigens, Bacterial/immunology
KW - Cattle/microbiology
KW - Interferon-gamma/metabolism
KW - Peptides/immunology
KW - Skin Tests
KW - Tuberculin Test
KW - Tuberculosis, Bovine/diagnosis
UR - https://advances.sciencemag.org/content/suppl/2019/07/15/5.7.eaax4899.DC1
UR - https://www.scopus.com/pages/publications/85069933007
U2 - 10.1126/sciadv.aax4899
DO - 10.1126/sciadv.aax4899
M3 - Article
C2 - 31328169
SN - 2375-2548
VL - 5
JO - Science advances
JF - Science advances
IS - 7
M1 - eaax4899
ER -
SDG 3 Good Health and Well-being