A demonstration of a 1:1 correspondence between chiasma frequency and recombination using a Lolium perenne/Festuca pratensis substitution

Julie King, Luned A. Roberts, Mike J. Kearsey, Huw M. Thomas, Ian P. King, R. Neil Jones, Lin Shiow-Fen Huang, Ian Peter Armstead, W. Gareth Morgan, Ian Philip King

Research output: Contribution to journalArticlepeer-review

41 Citations (SciVal)

Abstract

A single chromosome of the grass species Festuca pratensis has been introgressed into Lolium perenne to produce a diploid monosomic substitution line (2n 2x 14). The chromatin of F. pratensis and L. perenne can be distinguished by genomic in situ hybridization (GISH), and it is therefore possible to visualize the substituted F. pratensis chromosome in the L. perenne background and to study chiasma formation in a single marked bivalent. Recombination occurs freely in the F. pratensis/L. perenne bivalent, and chiasma frequency counts give a predicted map length for this bivalent of 76 cM. The substituted F. pratensis chromosome was also mapped with 104 EcoRI/Tru91 and HindIII/Tru91 amplified fragment length polymorphisms (AFLPs), generating a marker map of 81 cM. This map length is almost identical to the map length of 76 cM predicted from the chiasma frequency data. The work demonstrates a 1:1 correspondence between chiasma frequency and recombination and, in addition, the absence of chromatid interference across the Festuca and Lolium centromeres.
Original languageEnglish
Pages (from-to)307-314
Number of pages8
JournalGenetics
Volume161
Issue number1
Publication statusPublished - 01 May 2002

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