TY - JOUR
T1 - A DNA Barcode for land plants
AU - Hollingsworth , Peter M
AU - Forrest, Laura L.
AU - Spouge, John L.
AU - Hajibabaei, Mehrdad
AU - Ratnasingham, Sujeevan
AU - van der Bank, Michelle
AU - Chase, Mark W.
AU - Cowan, Robyn S.
AU - Erickson, David L.
AU - Fazekas, Aron J.
AU - Graham, Sean W.
AU - James, Karen E.
AU - Kim, Ki-Joong
AU - Kress, W. John
AU - Schneider, Harald
AU - van AlphenStahl, Jonathan
AU - Barrett, Spencer C. H.
AU - van den Berg, Cassio
AU - Bogarin, Diego
AU - Burgess, Kevin S.
AU - Cameron, Kenneth M.
AU - Carine, Mark
AU - Chacon, Juliana
AU - Clark, Alexandra
AU - Clarkson, James J.
AU - Conrad, Ferozah
AU - Devey, Dion S.
AU - Hedderson, Terry A. J.
AU - Ford, C. S.
AU - Hollingsworth, Michelle L.
AU - Husband, Brian C.
AU - Kelly, Laura J.
AU - Kesanakurti, Prasad R.
AU - Kim, Jung Sung
AU - Kim, Young-Dong
AU - Lahaye, Renaud
AU - Lee, Hae-Lim
AU - Long, David G.
AU - Madriñán, Santiago
AU - Maurin, Olivier
AU - Meusnier, Isabelle
AU - Newmaster, Steven G.
AU - Park, Chong-Wook
AU - Percy, Diana M.
AU - Petersen, Gitte
AU - Richardson, J. E.
AU - Salazar, Gerardo A.
AU - Savolainen, Vincent
AU - Seberg, Ole
AU - Wilkinson, M. J.
AU - Yi, Dong-Keun
AU - Little, Damon P.
N1 - Hollingsworth, P. M., Forrest, L. L., Spouge, J. L., Hajibabaei, M., Ratnasingham, S., van der Bank, M., Chase, M. W., Cowan, R.S., Erickson, D. L., Fazekas, A. J., Graham, S.W., James, K. E., Kim, K.-J., Kress, W. J., Schneider, H., van Alphen Stahl, J., Barrett, S. C. H., van den Berg, C., Bogarin, D., Burgess, K. S., Cameron, K. M., Carine, M., Chacon, J., Clark, A. C., Conrad, F., Devey, D., Clarkson, J., Ford, C. S., Hedderson, T. A. J., Hollingsworth, M. L., Husband, B. C., Kelly, L. J., Kesanakurti, P. R., Kim, J. S., Kim, Y. D., Lahaye, R., Lee H.-L., Long, D. G., Madrigan, S., Maurin, O., Meusnier, I., Newmaster, S. G, Park ,C.-W., Percy, D. M., Petersen, G., Richardson, J. E., Salazar, G. A., Savolainen, V., Seberg, O., Wilkinson, M. J., Yi, D.-K., Little, D. P. (2009). A DNA Barcode for land plants. Proceedings of the National Academy of Sciences of the USA, 106, (31), 12794-12797.
Sponsorship: Alfred P. Sloan Foundation, Gordon and Betty Moore Foundation, Genome Canada, Scottish Government's Rural and Environment Research and Analysis Directorate, Royal Society, South African National Research Foundation, Intramural Research Program of the National Library of Medicine, National Institutes of Health, and Consortium for the Barcode of Life.
PY - 2009
Y1 - 2009
N2 - DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
AB - DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
U2 - 10.1073/pnas.0905845106
DO - 10.1073/pnas.0905845106
M3 - Article
SN - 0027-8424
VL - 106
SP - 12794
EP - 12797
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 31
ER -