A Gene Encoding a DUF247 Domain Protein Cosegregates with the S Self-Incompatibility Locus in Perennial Ryegrass

Chloé Manzanares, Susanne Barth, Daniel Thorogood, Stephen Byrne, Steven Yates, Adrian Czaban, Torben Asp, Bicheng Yang, Bruno Studer

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The grass family (Poaceae), the fourth largest family of flowering plants, encompasses the most economically important cereal, forage, and energy crops, and exhibits a unique gametophytic self-incompatibility (SI) mechanism that is controlled by at least two multiallelic and independent loci, S and Z. Despite intense research efforts over the last six decades, the genes underlying S and Z remain uncharacterized. Here, we report a fine-mapping approach to identify the male component of the S-locus in perennial ryegrass (Lolium perenne L.) and provide multiple evidence that a domain of unknown function 247 (DUF247) gene is involved in its determination. Using a total of 10,177 individuals from seven different mapping populations segregating for S, we narrowed the S-locus to a genomic region containing eight genes, the closest recombinant marker mapping at a distance of 0.016 cM. Of the eight genes cosegregating with the S-locus, a highly polymorphic gene encoding for a protein containing a DUF247 was fully predictive of known S-locus genotypes at the amino acid level in the seven mapping populations. Strikingly, this gene showed a frameshift mutation in self-compatible darnel (Lolium temulentum L.), whereas all of the self-incompatible species of the Festuca–Lolium complex were predicted to encode functional proteins. Our results represent a major step forward toward understanding the gametophytic SI system in one of the most important plant families and will enable the identification of additional components interacting with the S-locus.
Original languageEnglish
Pages (from-to)870-884
Number of pages15
JournalMolecular Biology and Evolution
Issue number4
Early online date10 Dec 2015
Publication statusPublished - 01 Apr 2016


  • Domain of unknown function (DUF) 247
  • Fine-mapping
  • Perennial ryegrass (Lolium perenne L.)
  • Self-incompatibility (SI)
  • RNA sequencing (RNAseq)
  • S-locus
  • Perennial ryegrass (lolium perenne L.)
  • Selfincompatibility (SI)
  • Protein Kinases/genetics
  • Genotype
  • Chromosome Mapping
  • Plant Weeds/genetics
  • Plant Proteins/genetics
  • High-Throughput Nucleotide Sequencing
  • Self-Incompatibility in Flowering Plants/genetics
  • Genetic Linkage


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