TY - JOUR
T1 - A Gene Encoding a DUF247 Domain Protein Cosegregates with the S Self-Incompatibility Locus in Perennial Ryegrass
AU - Manzanares, Chloé
AU - Barth, Susanne
AU - Thorogood, Daniel
AU - Byrne, Stephen
AU - Yates, Steven
AU - Czaban, Adrian
AU - Asp, Torben
AU - Yang, Bicheng
AU - Studer, Bruno
N1 - This is the author accepted manuscript. The final version is available from Oxford University Press via http://dx.doi.org/10.1093/molbev/msv335
PY - 2015/12/10
Y1 - 2015/12/10
N2 - The grass family (Poaceae), the fourth largest family of flowering plants, encompasses the most economically important cereal, forage, and energy crops, and exhibits a unique gametophytic self-incompatibility (SI) mechanism that is controlled by at least two multiallelic and independent loci, S and Z. Despite intense research efforts over the last six decades, the genes underlying S and Z remain uncharacterized. Here, we report a fine-mapping approach to identify the male component of the S-locus in perennial ryegrass (Lolium perenne L.) and provide multiple evidence that a domain of unknown function 247 (DUF247) gene is involved in its determination. Using a total of 10,177 individuals from seven different mapping populations segregating for S, we narrowed the S-locus to a genomic region containing eight genes, the closest recombinant marker mapping at a distance of 0.016 cM. Of the eight genes cosegregating with the S-locus, a highly polymorphic gene encoding for a protein containing a DUF247 was fully predictive of known S-locus genotypes at the amino acid level in the seven mapping populations. Strikingly, this gene showed a frameshift mutation in self-compatible darnel (Lolium temulentum L.), whereas all of the self-incompatible species of the Festuca–Lolium complex were predicted to encode functional proteins. Our results represent a major step forward toward understanding the gametophytic SI system in one of the most important plant families and will enable the identification of additional components interacting with the S-locus.
AB - The grass family (Poaceae), the fourth largest family of flowering plants, encompasses the most economically important cereal, forage, and energy crops, and exhibits a unique gametophytic self-incompatibility (SI) mechanism that is controlled by at least two multiallelic and independent loci, S and Z. Despite intense research efforts over the last six decades, the genes underlying S and Z remain uncharacterized. Here, we report a fine-mapping approach to identify the male component of the S-locus in perennial ryegrass (Lolium perenne L.) and provide multiple evidence that a domain of unknown function 247 (DUF247) gene is involved in its determination. Using a total of 10,177 individuals from seven different mapping populations segregating for S, we narrowed the S-locus to a genomic region containing eight genes, the closest recombinant marker mapping at a distance of 0.016 cM. Of the eight genes cosegregating with the S-locus, a highly polymorphic gene encoding for a protein containing a DUF247 was fully predictive of known S-locus genotypes at the amino acid level in the seven mapping populations. Strikingly, this gene showed a frameshift mutation in self-compatible darnel (Lolium temulentum L.), whereas all of the self-incompatible species of the Festuca–Lolium complex were predicted to encode functional proteins. Our results represent a major step forward toward understanding the gametophytic SI system in one of the most important plant families and will enable the identification of additional components interacting with the S-locus.
KW - Domain of unknown function (DUF) 247
KW - Fine-mapping
KW - Perennial ryegrass (Lolium perenne L.)
KW - Self-incompatibility (SI)
KW - RNA sequencing (RNAseq)
KW - S-locus
UR - http://hdl.handle.net/2160/36436
UR - https://academic.oup.com/mbe/article/33/4/870/2579443#supplementary-data
U2 - 10.1093/molbev/msv335
DO - 10.1093/molbev/msv335
M3 - Article
C2 - 26659250
SN - 0737-4038
VL - 33
SP - 870
EP - 884
JO - Molecular Biology and Evolution
JF - Molecular Biology and Evolution
IS - 4
ER -