A genetic map of the apospory-region in Brachiaria hybrids: identification of two markers closely associated with the trait

Silvina C. Pessino, C. Evans, Juan Pablo A. Ortiz, Ian Armstead, Cacilda B. do Valle, Michael D. Hayward

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Abstract

The objective of this work was to identify molecular markers tightly linked to the gene controlling apospory in a hybrid population derived from a cross between apomictic Brachiaria brizantha and sexual Brachiaria ruziziensis. Since a maize chromosome 5 linkage group had previously been associated with the apomixis locus in Brachiaria we used twenty-five RFLP clones that map in detail maize chromosome 5 to cover systematically all syntenic regions of the hybrid genome. Forty-six AFLP markers were also generated. Three RFLP markers (detected by rz567, rz273, and cdo507 and two AFLP markers (PAM52-5 and PAM49-13) appeared to be related to the apo-region. Segregation data, together with previously reported data (corresponding to RFLP markers umc147, umc72, csu134, csu149 and RAPD marker OPC4) were used to generate a complete map of the region. Markers PAM52-5 and PAM49-13 were located respectively at 1.2 cM and 5.7 cM either side of the target locus. The map shows close synteny to regions of maize chromosome 5 and rice chromosome 2.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalHereditas
Volume128
Issue number2
DOIs
Publication statusPublished - 1998

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