TY - JOUR
T1 - A guinea pig model of low-dose Mycobacterium bovis aerogenic infection
AU - Chambers, Mark A.
AU - Williams, Ann
AU - Gavier-Widén, Dolores
AU - Whelan, Adam
AU - Hughes, Catherine
AU - Hall, Graham
AU - Lever, M. Stephen
AU - Marsh, Philip D.
AU - Hewinson, R. Glyn
PY - 2001/6/1
Y1 - 2001/6/1
N2 - In order to develop a model of Mycobacterium bovis infection with pathogenetical relevance, a modified version of the Henderson apparatus was used to deliver infectious aerosols directly to the snouts of guinea pigs. Aerosols generated from 106, 107, 108CFU/ml M. bovis suspensions established disease in every animal, with estimated retained doses of 10, 100, 1000CFU, respectively. For comparison, other guinea pigs were inoculated with 100CFU M. bovis intramuscularly (i.m.). Pathology and bacterial colonisation of lungs and spleen varied according to the dose and route of inoculation. Animals inoculated i.m. gave a significant cutaneous tuberculin hypersensitivity reaction earlier after testing than those infected aerogenically. A serological response to M. bovis antigens was detected in all infected animals. Intensity of antigen recognition was dose-dependent and although the range of antigens recognised varied between animals, a 25kDa antigen present in the cell fraction was serodominant. Thus, a reproducible guinea pig model has been defined that may be suitable for virulence, vaccination, and immunological studies.
AB - In order to develop a model of Mycobacterium bovis infection with pathogenetical relevance, a modified version of the Henderson apparatus was used to deliver infectious aerosols directly to the snouts of guinea pigs. Aerosols generated from 106, 107, 108CFU/ml M. bovis suspensions established disease in every animal, with estimated retained doses of 10, 100, 1000CFU, respectively. For comparison, other guinea pigs were inoculated with 100CFU M. bovis intramuscularly (i.m.). Pathology and bacterial colonisation of lungs and spleen varied according to the dose and route of inoculation. Animals inoculated i.m. gave a significant cutaneous tuberculin hypersensitivity reaction earlier after testing than those infected aerogenically. A serological response to M. bovis antigens was detected in all infected animals. Intensity of antigen recognition was dose-dependent and although the range of antigens recognised varied between animals, a 25kDa antigen present in the cell fraction was serodominant. Thus, a reproducible guinea pig model has been defined that may be suitable for virulence, vaccination, and immunological studies.
KW - Airborne infection
KW - Guinea pig model
KW - Mycobacterium bovis
UR - http://www.scopus.com/inward/record.url?scp=0035047143&partnerID=8YFLogxK
U2 - 10.1016/S0378-1135(00)00378-3
DO - 10.1016/S0378-1135(00)00378-3
M3 - Article
C2 - 11337137
AN - SCOPUS:0035047143
SN - 0378-1135
VL - 80
SP - 213
EP - 226
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3
ER -
