A low mutation rate for chloroplast microsatellites

Jim Provan, Nicole Soranzo, Neil J. Wilson, David B. Goldstein, W Powell

Research output: Contribution to journalArticlepeer-review

194 Citations (SciVal)

Abstract

We used chloroplast simple sequence repeats (cpSSRs) to examine whether there is any variation present in the chloroplast genome of Pinus torreyana (Parry ex Carriere) that may previously not have been detected using RFLPs. Analysis of 17 cpSSR loci showed no variation, which is consistent with previous cpRFLP work and confirms that the species is descended from an original, highly monomorphic population following a bottleneck. This lack of biological variation in the chloroplast genome of P. torreyana allowed us to estimate the mutation rates at cpSSR loci as between 3.2 x 10(-5) and 7.9 x 10(-5). This estimate is lower than published mutation rates at nuclear SSR loci but higher than substitution rates elsewhere in the chloroplast genome.

Original languageEnglish
Pages (from-to)943-947
Number of pages5
JournalGenetics
Volume153
Issue number2
Publication statusPublished - Oct 1999

Keywords

  • SIMPLE-SEQUENCE REPEATS
  • PINUS-TORREYANA
  • GENETIC DIVERSITY
  • POPULATIONS
  • GENOME
  • PINES

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