A lymphocyte transformation assay for the detection of Mycobacterium bovis infection in the Eurasian Badger (Meles meles)

D. Dalley, M. A. Chambers*, P. Cockle, W. Pressling, D. Gavier-Widén, R. G. Hewinson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

35 Citations (SciVal)

Abstract

The Eurasian badger (Meles meles) is a significant wildlife reservoir of Mycobacterium bovis in Great Britain. Improved control strategies against the disease in badgers require the development of diagnostic tests and vaccines. Here, we report the development of a comparative lymphocyte transformation assay (LTA) using bovine and avian tuberculin as antigen to detect cell-mediated responses in M. bovis-infected badgers. In a pilot study, the performance of this assay was compared with the existing indirect ELISA assay for the detection of tuberculous badgers. The sensitivity of the Comparative LTA was 87.5% compared with 62.5% for the indirect ELISA whereas the ELISA test gave a greater specificity (100% compared with 84.6% for the comparative LTA). Preliminary evidence suggests that for the comparative LTA, the blood may be stored overnight prior to testing and that this procedure might improve the specificity of the assay without compromising the sensitivity.

Original languageEnglish
Pages (from-to)85-94
Number of pages10
JournalVeterinary Immunology and Immunopathology
Volume70
Issue number1-2
DOIs
Publication statusPublished - 01 Sept 1999

Keywords

  • Assay
  • Badger
  • Immunology
  • Lymphocyte
  • Meles
  • Mycobacterium bovis
  • Bacterial Proteins/immunology
  • Carnivora
  • Enzyme-Linked Immunosorbent Assay/veterinary
  • Lymphocyte Activation
  • Antigens, Bacterial
  • Mycobacterium bovis/isolation & purification
  • United Kingdom/epidemiology
  • Membrane Proteins
  • Tuberculosis/diagnosis
  • Animals
  • Cattle
  • Reagent Kits, Diagnostic
  • Tuberculosis, Bovine/epidemiology
  • Mice
  • Disease Reservoirs
  • Antibodies, Bacterial/biosynthesis

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