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Abstract
Although a wealth of information is available on the induction of one or several drought related responses in different species, little is known of how their timing, modulation and crucially integration influence drought tolerance. Based on metabolomic changes in oat (Avena sativa L.) we have defined key processes involved in drought tolerance. During a time course of increasing water deficit, metabolites from leaf samples were profiled using Direct Infusion – Electrospray Mass Spectroscopy (DI-ESI-MS) and HPLC ESI-MS/MS and analysed using Principal Component Analysis and Discriminant Function Anlaysis. The involvement of metabolite pathways was confirmed through targeted assays of key metabolites and physiological experiments. We demonstrate an early accumulation of SA influencing stomatal opening, photorespiration and antioxidant defences before any change in relative water content. These changes are likely to maintain plant water status, with any photoinhibitory effect being counteracted by an efficient antioxidant capacity, thereby representing an integrated mechanism of drought tolerance in oats. We also discuss these changes in relation to those engaged at later points consequence of the different water status in susceptible and resistant genotypes.
Original language | English |
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Pages (from-to) | 1434-1452 |
Number of pages | 19 |
Journal | Plant, Cell and Environment |
Volume | 38 |
Issue number | 7 |
Early online date | 23 Dec 2014 |
DOIs | |
Publication status | Published - 31 Jul 2015 |
Keywords
- metabolomics
- photorespiration
- salicylic acid
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Dive into the research topics of 'A metabolomic study in oats (Avena sativa) highlights a drought tolerance mechanism based on salicylate signalling pathways and the modulation of carbon, antioxidant and photo-oxidative metabolism'. Together they form a unique fingerprint.Profiles
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Alison Kingston-Smith
- Faculty of Earth and Life Sciences, Institute of Biological, Environmental & Rural Sciences (IBERS) - Chair
Person: Teaching And Research
Projects
- 1 Finished
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RSB : Rumen Systems Biology
Biotechnology and Biological Sciences Research Council
01 Apr 2012 → 31 Mar 2017
Project: Externally funded research