A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

Kathryn Sinclair*, Janet A. Challans, Rudovick R. Kazwala, R. Glyn Hewinson, J. Michael Sharp

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

Original languageEnglish
Pages (from-to)291-295
Number of pages5
JournalMolecular and Cellular Probes
Volume9
Issue number5
DOIs
Publication statusPublished - 01 Oct 1995

Keywords

  • Diagnosis
  • M. tuberculosis complex
  • Polymerase chain reaction
  • Tuberculosis/diagnosis
  • Diagnosis, Differential
  • Base Sequence
  • Humans
  • Polymerase Chain Reaction
  • Molecular Sequence Data
  • Mycobacterium/isolation & purification
  • Mycobacterium tuberculosis/isolation & purification
  • DNA Primers

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