A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

Kathryn Sinclair; Janet A. Challans; Rudovick R. Kazwala; R. Glyn Hewinson; J. Michael Sharp

doi:10.1016/S0890-8508(95)91524-9

A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

Kathryn Sinclair^*, Janet A. Challans, Rudovick R. Kazwala, R. Glyn Hewinson, J. Michael Sharp

^*Corresponding author for this work

Bovine TB, diseases and control

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

Original language	English
Pages (from-to)	291-295
Number of pages	5
Journal	Molecular and Cellular Probes
Volume	9
Issue number	5
DOIs	https://doi.org/10.1016/S0890-8508(95)91524-9
Publication status	Published - 01 Oct 1995

Keywords

Diagnosis
M. tuberculosis complex
Polymerase chain reaction
Tuberculosis/diagnosis
Diagnosis, Differential
Base Sequence
Humans
Polymerase Chain Reaction
Molecular Sequence Data
Mycobacterium/isolation & purification
Mycobacterium tuberculosis/isolation & purification
DNA Primers

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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title = "A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex",

abstract = "A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.",

keywords = "Diagnosis, M. tuberculosis complex, Polymerase chain reaction, Tuberculosis/diagnosis, Diagnosis, Differential, Base Sequence, Humans, Polymerase Chain Reaction, Molecular Sequence Data, Mycobacterium/isolation & purification, Mycobacterium tuberculosis/isolation & purification, DNA Primers",

author = "Kathryn Sinclair and Challans, {Janet A.} and Kazwala, {Rudovick R.} and Hewinson, {R. Glyn} and Sharp, {J. Michael}",

year = "1995",

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T1 - A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

AU - Sinclair, Kathryn

AU - Challans, Janet A.

AU - Kazwala, Rudovick R.

AU - Hewinson, R. Glyn

AU - Sharp, J. Michael

PY - 1995/10/1

Y1 - 1995/10/1

N2 - A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

AB - A multiplex polymerase chain reaction has been developed which is able to distinguish Mycobacterium tuberculosis from other members of the M. tuberculosis complex. The assay is based on the simultaneous amplification of two different targets: a 396bp region from the mtp40 species-specific gene sequence of M. tuberculosis and a 245bp fragment from the M. tuberculosis complex insertion sequence IS986. Results have been obtained for 54 mycobacterial strains including five non-M. tuberculosis complex isolates. All 49 strains of the M. tuberculosis complex were positive for IS986 but only the 27 M. tuberculosis isolates were positive for both IS986 and mtp40.

KW - Diagnosis

KW - M. tuberculosis complex

KW - Polymerase chain reaction

KW - Tuberculosis/diagnosis

KW - Diagnosis, Differential

KW - Base Sequence

KW - Humans

KW - Polymerase Chain Reaction

KW - Molecular Sequence Data

KW - Mycobacterium/isolation & purification

KW - Mycobacterium tuberculosis/isolation & purification

KW - DNA Primers

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VL - 9

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IS - 5

ER -

A multiplex polymerase chain reaction for distinguishing Mycobacterium tuberculosis from Mycobacterium tuberculosis complex

Abstract

Keywords

UN SDGs

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