A new genetic locus for self-compatibility in the outcrossing grass species perennial ryegrass (Lolium perenne)

Lucy Marie Slatter, Susanne Barth, Chloé Manzanares, Janaki Velmurugan, Iain Place, Danny Thorogood

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Abstract

Background
Self-incompatibility (SI) is a physiological mechanism that many flowering plants employ to prevent self-fertilization and maintain heterozygosity. In the grass family this is known to be controlled by a two locus (S-Z) system; however, the SI system is intrinsically leaky. Modifier genes of both the S and Z loci and a further locus, T, are known to override SI leading to self-fertilization and self-seed production. This has implications for the ecological and evolutionary success as well as the commercial breeding of grasses. Here we report a study where the genetic control of self-compatibility (SC) was determined from the results of self-pollinating an F2 population of perennial ryegrass from two independently derived inbred lines produced by single-seed descent.

Methods
In vitro self-pollinations of 73 fertile plants were analysed. A genetic association analysis was made with a panel of 1863 single-nucleotide polymorphism (SNP) markers, generated through genotype-by-sequencing methodology. Markers were placed on a recombination map of seven linkage groups (LGs) created using Joinmap v.5. The seed set on self- and open-pollinated inflorescences was determined on 143 plants, including the 73 plants analysed for self-pollination response.

Key Results
Self-pollinations revealed a bimodal distribution of percentage SC with peaks at 50 and 100 %. A single quantitative trait locus (QTL) was identified with peak association for marker 6S14665z17875_11873 that mapped to LG 6. Peak position was associated with maximum marker segregation distortion. The self-compatible plants were equally fecund after self- and open pollination.

Conclusions
This is the first report in the Poaceae family of an SC locus located on LG 6. This new SC QTL discovery, as well as indicating the complex nature of the pollen–stigma recognition process and its evolutionary significance, provides an additional source of SC for breeding perennial ryegrass.
Original languageEnglish
Article numbermcaa140
Pages (from-to)715-722
Number of pages8
JournalAnnals of Botany
Volume127
Issue number6
Early online date28 Aug 2020
DOIs
Publication statusPublished - 07 May 2021

Keywords

  • Keywords. genetic linkage map, genotyping by sequencing, heterosis, inbreeding, Poaceae, quantitative trait locus, reproductive assurance, self-incompatibility, single nucleotide polymorphism
  • genotyping by sequencing
  • reproductive assurance
  • Genetic linkage map
  • heterosis
  • self-incompatibility
  • Poaceae
  • quantitative trait locus
  • inbreeding
  • single-nucleotide polymorphism
  • Lolium/genetics
  • Plant Breeding
  • Chromosome Mapping
  • Genetic Linkage

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