A pink bacterium as a reporter system signaling expression of a recombinant protein

N. Kaderbhai, J. Gallagher, M. He, M. A. Kaderbhai

Research output: Contribution to journalArticlepeer-review

Abstract

We describe a set of expression vectors, pEX-PINK/0–3, for high-level production of (un)fused target proteins. The vectors incorporate a ‘pink’ reporter element, which signals in vivo the expression status of a target gene. A target sequence is cloned between the λ P L promoter and the downstream mammalian cytochrome b 5 gene. Thermo-induction drives transcription of a dicistronic mRNA from which the target protein and cytochrome b 5 are independently and concurrently synthesized. Positive expression is indicated by visual transformation of bacteria from a grey/translucent to a bright pink color derived from tandemly expressed holocytochrome b 5. The signal can be monitored in vivo spectrophotometrically.

Original languageEnglish
Pages (from-to)567-577
Number of pages11
JournalDNA and Cell Biology
Volume11
Issue number7
DOIs
Publication statusPublished - Sept 1992

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