A proposal for a standardised protocol to barcode all land plants

Mark W. Chase, Robyn S. Cowan, Peter M Hollingsworth , Cassio van den Berg, Santiago Madriñán, Gitte Peterson, Ole Seberg, Tina Jørgsensen, Kenneth M. Cameron, Mark Carine, Niklas Pederson, Terry A. J. Hedderson, Ferozah Conrad, Gerardo A. Salazar, James E. Richardson, Michelle L. Hollingsworth, Tim Barraclough, Laura Kelly, Michael J. Wilkinson

Research output: Contribution to journalArticlepeer-review

485 Citations (SciVal)

Abstract

We propose in this paper to use three regions of plastid DNA as a standard protocol for barcoding all land plants. We review the other markers that have been proposed and discuss their advantages and disadvantages. The low levels of variation in plastid DNA make three regions necessary; there are no plastid regions, coding or non-coding, that evolve as rapidly as mitochondrial DNA generally does in animals. We outline two, three-region options, (1) rpoC1, rpoB and matK or (2) rpoC1, matK and psbA-trnH as viable markers for land plant barcoding.
Original languageEnglish
Pages (from-to)295-299
Number of pages5
JournalTaxon
Volume56
Issue number2
Publication statusPublished - May 2007

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