A Proteomics Approach To Quantify Protein Levels Following RNA Interference: Case Study with Glutathione Transferase Superfamily from the Model Metazoan Caenorhabditis elegans

E. J. LaCourse, Samirah Perally, M. Hernandez-Viadel, Hazel A. Wright, Peter M. Brophy

Research output: Contribution to journalArticlepeer-review

11 Citations (SciVal)

Abstract

Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations; protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.
Original languageEnglish
Pages (from-to)3314-3318
Number of pages5
JournalJournal of Proteome Research
Volume7
Issue number8
DOIs
Publication statusPublished - 27 Jun 2008

Keywords

  • RNAi
  • Glutathione transferase
  • Caenorhabditis elegans

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