@article{3135c857d1a64134b63b65cc93317ff6,
title = "A Proteomics Approach To Quantify Protein Levels Following RNA Interference: Case Study with Glutathione Transferase Superfamily from the Model Metazoan Caenorhabditis elegans",
abstract = "Loss-of-function phenotypic analysis via interference RNA (RNAi) technology is a revolutionary approach to assigning gene function. While transcript-based methodologies commonly validate RNAi gene suppression investigations; protein-based validation is less developed. This report illustrates the potential for two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis (2-DE) and gel analysis to quantify protein levels following RNAi. This case study involves three glutathione transferase (GST) genes targeted by RNAi from the model organism Caenorhabditis elegans.",
keywords = "RNAi, Glutathione transferase, Caenorhabditis elegans",
author = "LaCourse, {E. J.} and Samirah Perally and M. Hernandez-Viadel and Wright, {Hazel A.} and Brophy, {Peter M.}",
note = "LaCourse, E. J., Perally, S., Hernandez-Viadel, M. Wright, H. A., Brophy, P. M. (2008). A proteomics approach to quantify protein levels following RNA interference: case study with glutathione transferase superfamily from the model metazoan Caenorhabditis elegans. Journal of Proteome Research, 7, (8), pp. 3314-3318 Keywords: RNAi • Glutathione transferase • Caenorhabditis elegans C. elegans is a functional genomics model for studying related parasitic nematodes of veterinary importance. The paper showed the for the first time the application of proteomics to validate the outcome of RNAi knockdown in the model (Prot.RNAi), and showed for the first time evidence of genetic buffering ) in a protein superfamily (increased expression of family member on knockdown of another member). The major contributor to experimental design and manuscript write-up and NERC grant holder/PhD supervisor (JLaC) at Aberystwyth. On file IMPF: 05.68 ",
year = "2008",
month = jun,
day = "27",
doi = "10.1021/pr8001035",
language = "English",
volume = "7",
pages = "3314--3318",
journal = "Journal of Proteome Research",
issn = "1535-3893",
publisher = "American Chemical Society",
number = "8",
}