A reformulation of Murashige and Skoog medium (WPBS medium) improves embryogenesis, morphogenesis and transformation efficiency in temperate and tropical grasses and cereals

Sue Dalton

Research output: Contribution to journalArticlepeer-review

7 Citations (SciVal)
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Abstract

Slow callus growth is a barrier to efficient genetic transformation in some gramineous species. A reformulation of Murashige and Skoog (MS) medium, with additional magnesium sulphate, potassium phosphate, copper sulphate, proline and glutamine, termed WPBS medium, has been developed which improves all aspects of in vitro culture when compared with MS based media. Embryogenic callus could be produced more rapidly from responsive genotypes of sixteen cereal, forage, model and energy grass species, whether using embryos, shoot tips or proliferated meristems as explants. Three species were not transformed due to contamination or unsuitable explant, but thirteen species were transformed using an identical Agrobacterium-mediated transformation, selection and regeneration protocol, including Avena sativa and Oryza sativa. Readily transformable species such as Lolium perenne, Brachypodium distachyon and Festuca arundinacea and recalcitrant species such as Lolium temulentum and Miscanthus sinensis were reliably transformed, while two new species Phalaris arundinacea and viviparous Deschampsia cespitosa were transformed at the first attempt. It is hoped that the use of WPBS media and this general transformation protocol may help to improve the efficiency of grass and cereal transformation.
Original languageEnglish
Pages (from-to)257-273
Number of pages17
JournalPlant Cell, Tissue and Organ Culture
Volume141
Issue number2
Early online date19 Feb 2020
DOIs
Publication statusPublished - 01 May 2020

Keywords

  • embryogenic callus
  • transformation
  • forage grasses
  • model grasses
  • energy grasses
  • cereals
  • Transformation
  • Cereals
  • Model grasses
  • Forage grasses
  • Embryogenic callus
  • Energy grasses

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