A secondary effect of transformation in Rhizobium leguminosarum transgenic for Bacillus thuringiensis subspecies tenebrionis δ-endotoxin (cryIIIA) genes

Glynis Giddings, Lance Mytton, M. Griffiths, A. McCarthy, C. Morgan, Leif Skot

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

By introducing Bacillus thuringiensis subspecies tenebrionisδ-endotoxin genes (cryIIIA) into Rhizobium leguminosarum we have produced strains for the biological control of Sitona larvae. Comparisons between a transgenic and the parent strain show that transformation has induced changes not associated with the intended function of the transgene. Although growth rates in laboratory cultures are similar for both strains, the ability to compete for nodule occupancy is greater in the transgenic than in the non-transformed parent strain. This result demonstrates the importance of studying ecological and agronomic characters of transgenic micro-organisms that could have a bearing on the safety and success of their release into the environment, even if they are not thought to be connected with the transgenes introduced.
Original languageEnglish
Pages (from-to)1062-1068
Number of pages7
JournalTheoretical and Applied Genetics
Volume95
Issue number7
DOIs
Publication statusPublished - Nov 1997

Keywords

  • Nodule occupancy
  • Rhizobium
  • Transformation

Fingerprint

Dive into the research topics of 'A secondary effect of transformation in Rhizobium leguminosarum transgenic for Bacillus thuringiensis subspecies tenebrionis δ-endotoxin (cryIIIA) genes'. Together they form a unique fingerprint.

Cite this