A simple and rapid method for the analysis of water soluble carbohydrates from small segments of cereal leaf tissue

J. Harrison; Joseph Gallagher; Christopher Pollock

doi:10.1016/S0176-1617(97)80061-2

A simple and rapid method for the analysis of water soluble carbohydrates from small segments of cereal leaf tissue

J. Harrison, Joseph Gallagher, Christopher Pollock

Department of Life Sciences

Institute of Grassland and Environmental Research

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A simple three-step assay has been developed for the analysis of nanomole quantities of glucose, fructose and sucrose from small plant samples in the presence of fructans. The assay of crude ethanol/water extracts proceeds by the sequential addition of the commercially available enzymes; hexokinase, phosphogluco-isomerase, and sucrose Phosphorylase. The assay was compared with conventional coupled assays for glucose and fructose, together with TLC and HPLC assays for total water-soluble carbohydrates. All assays showed similar results over the range of standards and plant samples tested. The assay has been developed for use on microtiter plate readers, allowing for the screening of large numbers of samples rapidly. Fructan content in the same plant samples can be calculated following the determination of total free and combined hexoses.

Original language	English
Pages (from-to)	654-659
Number of pages	6
Journal	Journal of Plant Physiology
Volume	151
Issue number	6
DOIs	https://doi.org/10.1016/S0176-1617(97)80061-2
Publication status	Published - 1997

Keywords

Carbohydrates
Fructan
Fructose
Glucose
Leaves
Plants
Sucrose

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10.1016/S0176-1617(97)80061-2

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title = "A simple and rapid method for the analysis of water soluble carbohydrates from small segments of cereal leaf tissue",

abstract = "A simple three-step assay has been developed for the analysis of nanomole quantities of glucose, fructose and sucrose from small plant samples in the presence of fructans. The assay of crude ethanol/water extracts proceeds by the sequential addition of the commercially available enzymes; hexokinase, phosphogluco-isomerase, and sucrose Phosphorylase. The assay was compared with conventional coupled assays for glucose and fructose, together with TLC and HPLC assays for total water-soluble carbohydrates. All assays showed similar results over the range of standards and plant samples tested. The assay has been developed for use on microtiter plate readers, allowing for the screening of large numbers of samples rapidly. Fructan content in the same plant samples can be calculated following the determination of total free and combined hexoses.",

keywords = "Carbohydrates, Fructan, Fructose, Glucose, Leaves, Plants, Sucrose",

author = "J. Harrison and Joseph Gallagher and Christopher Pollock",

note = "Funding Information: We acknowledge the financial support from the BBSRC BAGEC program during the execution of these studies.",

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doi = "10.1016/S0176-1617(97)80061-2",

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T1 - A simple and rapid method for the analysis of water soluble carbohydrates from small segments of cereal leaf tissue

AU - Harrison, J.

AU - Gallagher, Joseph

AU - Pollock, Christopher

N1 - Funding Information: We acknowledge the financial support from the BBSRC BAGEC program during the execution of these studies.

PY - 1997

Y1 - 1997

N2 - A simple three-step assay has been developed for the analysis of nanomole quantities of glucose, fructose and sucrose from small plant samples in the presence of fructans. The assay of crude ethanol/water extracts proceeds by the sequential addition of the commercially available enzymes; hexokinase, phosphogluco-isomerase, and sucrose Phosphorylase. The assay was compared with conventional coupled assays for glucose and fructose, together with TLC and HPLC assays for total water-soluble carbohydrates. All assays showed similar results over the range of standards and plant samples tested. The assay has been developed for use on microtiter plate readers, allowing for the screening of large numbers of samples rapidly. Fructan content in the same plant samples can be calculated following the determination of total free and combined hexoses.

AB - A simple three-step assay has been developed for the analysis of nanomole quantities of glucose, fructose and sucrose from small plant samples in the presence of fructans. The assay of crude ethanol/water extracts proceeds by the sequential addition of the commercially available enzymes; hexokinase, phosphogluco-isomerase, and sucrose Phosphorylase. The assay was compared with conventional coupled assays for glucose and fructose, together with TLC and HPLC assays for total water-soluble carbohydrates. All assays showed similar results over the range of standards and plant samples tested. The assay has been developed for use on microtiter plate readers, allowing for the screening of large numbers of samples rapidly. Fructan content in the same plant samples can be calculated following the determination of total free and combined hexoses.

KW - Carbohydrates

KW - Fructan

KW - Fructose

KW - Glucose

KW - Leaves

KW - Plants

KW - Sucrose

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DO - 10.1016/S0176-1617(97)80061-2

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SN - 0176-1617

VL - 151

SP - 654

EP - 659

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

IS - 6

ER -

A simple and rapid method for the analysis of water soluble carbohydrates from small segments of cereal leaf tissue

Abstract

Keywords

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