A three-dimensional model of primary bovine endometrium using an electrospun scaffold

S. B. MacKintosh, L. P. Serino, P. D. Iddon, R. Brown, R. S. Conlan, C. J. Wright, T. G. G. Maffeis, M. J. Raxworthy, I. M. Sheldon

Research output: Contribution to journalArticlepeer-review

24 Citations (SciVal)
167 Downloads (Pure)


Endometrial stromal and epithelial cell function is typically studied in vitro using standard two-dimensional monocultures, but these cultures fail to reflect the complex three-dimensional (3D) architecture of tissue. A 3D model of bovine endometrium that reflects the architectural arrangement of in vivo tissue would beneficially assist the study of tissue function. An electrospun polyglycolide (PGA) scaffold was selected to grow a 3D model of primary bovine endometrial epithelial and stromal cells, that reflects the architecture of the endometrium for the study of pathophysiology. Electrospun scaffolds were seeded with stromal and epithelial cells, and growth was assessed using histological techniques. Prostaglandin E2 and prostaglandin F2α responsiveness of endometrial scaffold constructs was tested using oxytocin plus arachidonic acid (OT + AA) or lipopolysaccharide (LPS). Stromal and epithelial cells growing on the electrospun scaffold had an architectural arrangement that mimicked whole tissue, deposited fibronectin, had appropriate expression of vimentin and cytokeratin and were responsive to OT + AA and LPS, as measured by prostaglandin accumulation. In conclusion, a functional 3D model of stromal and epithelial cells was developed using a PGA electrospun scaffold which may be used to study endometrial pathophysiology.
Original languageEnglish
Article number025010
Issue number2
Publication statusPublished - 28 May 2015


Dive into the research topics of 'A three-dimensional model of primary bovine endometrium using an electrospun scaffold'. Together they form a unique fingerprint.

Cite this