A xylanase produced by the rumen anaerobic protozoan Polyplastron multivesiculatum shows close sequence similarity to family 11 xylanases from Gram-positive bacteria

Estelle Devillard, C. J. Newbold, Karen P. Scott, Evelyne Forano, R. John Wallace, Jean-Pierre Jouany, Harry J. Flint

Research output: Contribution to journalArticlepeer-review

52 Citations (Scopus)

Abstract

We report for the first time the cloning and characterisation of a protozoal enzyme involved in plant cell wall polysaccharide degradation. A cDNA library was constructed from the ruminal protozoan Polyplastron multivesiculatum and a stable clone expressing xylanase activity was isolated. The encoded enzyme belongs to the glycoside hydrolase family 11, and phylogenetic analysis indicates a closer relationship with catalytic domains from Gram-positive bacteria than the other fibrolytic eukaryotes from the rumen, the anaerobic fungi.
Original languageEnglish
Pages (from-to)145-152
Number of pages8
JournalFEMS Microbiology Letters
Volume181
Issue number1
Early online date15 Nov 1999
DOIs
Publication statusPublished - 01 Dec 1999

Keywords

  • POPULATIONS
  • PROTEIN
  • rumen
  • CELLULOLYTIC BACTERIA
  • protozoa
  • CELLULASES
  • GENE
  • FUNGI
  • DIGESTION
  • ENZYMES
  • Polyplastron multivesiculatum
  • DOMAINS
  • SHEEP
  • xylanase
  • Protozoa
  • Rumen
  • Xylanase

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