TY - JOUR
T1 - Abundance of H‐2 promiscuous T cells specific for mycobacterial determinants in H‐2b/d F1 hybrid mice
AU - Vordermeier, H. Martin
AU - Arya, Anu
AU - Harris, David P.
AU - Moreno, Carlos
AU - Ivanyi, Juraj
PY - 1995/10/1
Y1 - 1995/10/1
N2 - A majority of immunodominant epitopes of mycobacterial antigens are known to be recognized by murine T cells in the context of several H‐2 haplotypes. In this study, we established the frequency of T cells able to recognize these peptides promiscuously, i.e. in the context of allogeneic antigen‐presenting cells, using hybridomas from peptide‐immunized H‐2 homologous and heterologous mice. The degree of promiscuity in homozygous mice varied between 4–27% between different specificities and genetic backgrounds. In particular, the results showed that promiscuity between Ab and Ad in respect to a peptide from the Mycobacterium tuberculosis 38‐kDa protein (residues 350–369) was displayed by 22% of BALB/c and 4% of C57BL/10‐derived hybrids, but by 42% of [BALB/c x C57BL/10] F1‐derived clones. This represents a significant increase (p < 0.001) of T cell promiscuity compared to the parental haplotypes. It is noteworthy that considerably lower peptide concentrations were able to stimulate the promiscuous hybridomas compared to the H‐2‐restricted hybrids. This finding suggests a functional advantage of promiscuous T cells which enables them to expand preferentially in the initial stages of infections with M. tuberculosis and thus enables the host to mount a rapid protective immune response.
AB - A majority of immunodominant epitopes of mycobacterial antigens are known to be recognized by murine T cells in the context of several H‐2 haplotypes. In this study, we established the frequency of T cells able to recognize these peptides promiscuously, i.e. in the context of allogeneic antigen‐presenting cells, using hybridomas from peptide‐immunized H‐2 homologous and heterologous mice. The degree of promiscuity in homozygous mice varied between 4–27% between different specificities and genetic backgrounds. In particular, the results showed that promiscuity between Ab and Ad in respect to a peptide from the Mycobacterium tuberculosis 38‐kDa protein (residues 350–369) was displayed by 22% of BALB/c and 4% of C57BL/10‐derived hybrids, but by 42% of [BALB/c x C57BL/10] F1‐derived clones. This represents a significant increase (p < 0.001) of T cell promiscuity compared to the parental haplotypes. It is noteworthy that considerably lower peptide concentrations were able to stimulate the promiscuous hybridomas compared to the H‐2‐restricted hybrids. This finding suggests a functional advantage of promiscuous T cells which enables them to expand preferentially in the initial stages of infections with M. tuberculosis and thus enables the host to mount a rapid protective immune response.
KW - Genetic restriction
KW - Mycobacteria
KW - Peptide epitopes
KW - T cells
UR - http://www.scopus.com/inward/record.url?scp=0028871331&partnerID=8YFLogxK
U2 - 10.1002/eji.1830251009
DO - 10.1002/eji.1830251009
M3 - Article
C2 - 7589070
AN - SCOPUS:0028871331
SN - 0014-2980
VL - 25
SP - 2770
EP - 2774
JO - European Journal of Immunology
JF - European Journal of Immunology
IS - 10
ER -