African 1, an epidemiologically important clonal complex of mycobacterium bovis dominant in Mali, Nigeria, Cameroon, and Chad

Borna Müller, Markus Hilty, Stefan Berg, M. Carmen Garcia-Pelayo, James Dale, M. Laura Boschiroli, Simeon Cadmus, Bongo Naré Richard Ngandolo, Sylvain Godreuil, Colette Diguimbaye-Djaibé, Rudovick Kazwala, Bassirou Bonfoh, Betty M. Njanpop-Lafourcade, Naima Sahraoui, Djamel Guetarni, Abraham Aseffa, Meseret H. Mekonnen, Voahangy Rasolofo Razanamparany, Herimanana Ramarokoto, Berit DjønneJames Oloya, Adelina Machado, Custodia Mucavele, Eystein Skjerve, Francoise Portaels, Leen Rigouts, Anita Michel, Annélle Müller, Gunilla Källenius, Paul D. Van Helden, R. Glyn Hewinson, Jakob Zinsstag, Stephen V. Gordon, Noel H. Smith

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92 Citations (SciVal)

Abstract

We have identified a clonal complex of Mycobacterium bovis present at high frequency in cattle in population samples from several sub-Saharan west-central African countries. This closely related group of bacteria is defined by a specific chromosomal deletion (RDAfl) and can be identified by the absence of spacer 30 in the standard spoligotype typing scheme. We have named this group of strains the African 1 (Afl) clonal complex and have defined the spoligotype signature of this clonal complex as being the same as the M. bovis BCG vaccine strain but with the deletion of spacer 30. Strains of the Afl clonal complex were found at high frequency in population samples of M. bovis from cattle in Mali, Cameroon, Nigeria, and Chad, and using a combination of variable-number tandem repeat typing and spoligotyping, we show that the population of M. bovis in each of these countries is distinct, suggesting that the recent mixing of strains between countries is not common in this area of Africa. Strains with the Afl-specific deletion (RDAfl) were not identified in M. bovis isolates from Algeria, Burundi, Ethiopia, Madagascar, Mozambique, South Africa, Tanzania, and Uganda. Furthermore, the spoligotype signature of the Afl clonal complex has not been identified in population samples of bovine tuberculosis from Europe, Iran, and South America. These observations suggest that the Afl clonal complex is geographically localized, albeit to several African countries, and we suggest that the dominance of the clonal complex in this region is the result of an original introduction into cows naïve to bovine tuberculosis.

Original languageEnglish
Pages (from-to)1951-1960
Number of pages10
JournalJournal of Bacteriology
Volume191
Issue number6
Early online date26 Feb 2009
DOIs
Publication statusPublished - 01 Mar 2009

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