Ag85A-specific CD4+ T cell lines derived after boosting BCG-vaccinated cattle with Ad5-85A possess both mycobacterial growth inhibition and anti-inflammatory properties

Hannah J. Metcalfe, Lucia Biffar, Sabine Steinbach, Efrain Guzman, Tim Connelley, Ivan Morrison, H. Martin Vordermeier, Bernardo Villarreal-Ramos*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (SciVal)
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Abstract

There is a need to improve the efficacy of the BCG vaccine against human and bovine tuberculosis. Previous data showed that boosting bacilli Calmette-Guerin (BCG)-vaccinated cattle with a recombinant attenuated human type 5 adenovirally vectored subunit vaccine (Ad5-85A) increased BCG protection and was associated with increased frequency of Ag85A-specific CD4+ T cells post-boosting. Here, the capacity of Ag85A-specific CD4+ T cell lines – derived before and after viral boosting – to interact with BCG-infected macrophages was evaluated. No difference before and after boosting was found in the capacity of these Ag85A-specific CD4+ T cell lines to restrict mycobacterial growth, but the secretion of IL-10 in vitro post-boost increased significantly. Furthermore, cell lines derived post-boost had no statistically significant difference in the secretion of pro-inflammatory cytokines (IL-1β IL-12, IFNγ or TNFα) compared to pre-boost lines. In conclusion, the protection associated with the increased number of Ag85A-specific CD4+ T cells restricting mycobacterial growth may be associated with anti-inflammatory properties to limit immune-pathology.

Original languageEnglish
Pages (from-to)2850-2854
Number of pages5
JournalVaccine
Volume36
Issue number20
Early online date11 Apr 2018
DOIs
Publication statusPublished - 11 May 2018

Keywords

  • Antigen specific
  • Cytokines
  • Mycobacteria killing
  • T-cell lines

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