Agrobacterium-mediated transformation of bread and durum wheat using freshly isolated immature embryos

Huixia Wu, Angela Doherty, Huw D Jones

Research output: Chapter in Book/Report/Conference proceedingChapter

18 Citations (SciVal)

Abstract

Agrobacterium-mediated transformation of wheat is becoming a viable alternative to the more established biolistic protocols. It offers advantages in terms of simple, low-copy-number integrations and can be applied with similar efficiencies to specific durum wheat and spring and winter bread wheat types varieties.
Original languageEnglish
Title of host publicationTransgenic Whaeat, Barley and Oats
Subtitle of host publicationProduction and Characterization Protocols
EditorsHuw D. Jones, Peter R. Shewry
PublisherSpringer Nature
Pages93-103
Number of pages11
Edition2
ISBN (Electronic)978-1-59745-379-0
ISBN (Print)978-1-58829-961-1
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume478
ISSN (Print)1064-3745

Keywords

  • Agrobacterium tumefaciens
  • bread wheat
  • durum wheat
  • AGL1
  • inoculation
  • immature embryo

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