Alignment of the genomes of brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization

Iain S. Donnison, Ian P. King, Elzbieta Wolny, Glyn M. Jenkins, Agnieszka Marasek, Ian P. Armstead, Robert Hasterok, Dominika Idziak, John H. Draper, Ann M. Thomas

Research output: Contribution to journalArticlepeer-review

106 Citations (SciVal)

Abstract

As part of an initiative to develop Brachypodium distachyon as a genomic 'bridge' species between rice and the temperate cereals and grasses, a BAC library has been constructed for the two diploid (2n = 2x = 10) genotypes, ABR1 and ABR5. The library consists of 9100 clones, with an approximate average insert size of 88 kb, representing 2.22 genome equivalents. To validate the usefulness of this species for comparative genomics and gene discovery in its larger genome relatives, the library was screened by PCR using primers designed on previously mapped rice and Poaceae sequences. Screening indicated a degree of synteny between these species and B. distachyon, which was confirmed by fluorescent in situ hybridization of the marker-selected BACs (BAC landing) to the 10 chromosome arms of the karyotype, with most of the BACs hybridizing as single loci on known chromosomes. Contiguous BACs colocalized on individual chromosomes, thereby confirming the conservation of genome synteny and proving that B. distachyon has utility as a temperate grass model species alternative to rice.
Original languageEnglish
Pages (from-to)349-362
Number of pages14
JournalGenetics
Volume173
Issue number1
DOIs
Publication statusPublished - 01 May 2006

Fingerprint

Dive into the research topics of 'Alignment of the genomes of brachypodium distachyon and temperate cereals and grasses using bacterial artificial chromosome landing with fluorescence in situ hybridization'. Together they form a unique fingerprint.

Cite this