An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

Michael Shemko, Zack Fang, Graham MacIntire, Royston Goodacre, Nandini Shetty, Vanya Gant, Yankuba Kassama

Research output: Contribution to journalArticlepeer-review

Abstract

We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of >65 mol% (4). By contrast, BamHI and MspI restriction endonucleases were used in this study because they have a higher cleavage frequency (as judged by in silico calculations) for the M. tuberculosis genome and do not show polymorphisms within IS6110/IS986
Original languageEnglish
Pages (from-to)288-289
Number of pages2
JournalJournal of Clinical Microbiology
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2006

Fingerprint

Dive into the research topics of 'An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis'. Together they form a unique fingerprint.

Cite this