An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

Michael Shemko; Zack Fang; Graham MacIntire; Royston Goodacre; Nandini Shetty; Vanya Gant; Yankuba Kassama

doi:10.1128/JCM.44.1.288-289.2006

An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

Michael Shemko
, Zack Fang
, Graham MacIntire
, Royston Goodacre
, Nandini Shetty
, Vanya Gant
, Yankuba Kassama

Institute of Biological, Environmental, and Rural Sciences

Institute of Environmental Science and Research
Kerala Institute of Medical Sciences
Health Protection Agency

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of >65 mol% (4). By contrast, BamHI and MspI restriction endonucleases were used in this study because they have a higher cleavage frequency (as judged by in silico calculations) for the M. tuberculosis genome and do not show polymorphisms within IS6110/IS986

Original language	English
Pages (from-to)	288-289
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	44
Issue number	1
DOIs	https://doi.org/10.1128/JCM.44.1.288-289.2006
Publication status	Published - Jan 2006

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abstract = "We have evaluated fluorescent amplified fragment length polymorphism (FAFLP) fingerprinting as a complementary technique for genotyping Mycobacterium tuberculosis, which may aid in the elucidation of the transmission dynamics of tuberculosis. Earlier FAFLP studies (1, 2, 3, 5) broadly employed EcoRI and MseI restriction enzymes, which are known to have a low cleavage frequency for GC genomes of >65 mol\% (4). By contrast, BamHI and MspI restriction endonucleases were used in this study because they have a higher cleavage frequency (as judged by in silico calculations) for the M. tuberculosis genome and do not show polymorphisms within IS6110/IS986",

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AU - Shemko, Michael

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AU - MacIntire, Graham

AU - Goodacre, Royston

AU - Shetty, Nandini

AU - Gant, Vanya

AU - Kassama, Yankuba

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An improved fluorescent amplified fragment length polymorphism method for typing Mycobacterium tuberculosis

Abstract

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