An off-the-shelf, authentic and versatile undergraduate molecular biology practical course.

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Abstract

We provide a prepackaged molecular biology course, which has a broad context and is scalable to large numbers of students. It is provided complete with technical setup guidance, a reliable assessment regime, and can be readily implemented without any development necessary. Framed as a forensic examination of blue/white cloning plasmids, the course is a versatile workbench, adaptable to different degree subjects, and can be easily modified to undertake novel research as part of its teaching activities. Course activities include DNA extraction, RFLP, PCR, DNA sequencing, gel electrophoresis, and transformation, alongside a range of basic microbiology techniques. Students particularly appreciated the relevance of the practical to professional practice and the authenticity of the experimental work. © 2015 by The International Union of Biochemistry and Molecular Biology, 2015
Original languageEnglish
Pages (from-to)434-440
Number of pages7
JournalBiochemistry and Molecular Biology Education
Volume43
Issue number6
Early online date30 Oct 2015
DOIs
Publication statusPublished - 14 Nov 2015

Keywords

  • education
  • practical course
  • molecular biology
  • bacterial genetics

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