An SSR and AFLP molecular marker-based genetic map of white clover (Trifolium repens L.)

Elizabeth S. Jones, Leonie J. Hughes, Michelle C. Drayton, Michael T. Abberton, Terry P. T. Michaelson-Yeates, Charlotte Bowen, John W. Forster

Research output: Contribution to journalArticlepeer-review


A framework genetic map of white clover (Trifolium repens L.) has been constructed using an F2 progeny set derived from the intercross of fourth and fifth generation inbred genotypes carrying a self-fertile mutation (Sf). White clover SSR (TRSSR) and AFLP markers were used to derive a map with 135 markers (78 TRSSR loci and 57 AFLP loci) assigned to 18 linkage groups (LGs) with a total map length of 825 cm. Sixteen of these LGs are presumed to correspond to the 16 chromosomes of the white clover karyotype. A limited number of multiple loci were detected. Substantial segregation distortion was observed for both mapped and unmapped loci, with a bias towards heterozygous types and a preponderance of distorted markers on certain LGs. This observation, along with a high degree of residual heterozygosity within the inbred parental genotypes, suggests that reduced individual fitness due to loss of heterosis is a major effect for white clover and will limit the applicability of F2 or backcross mapping strategies for this species. A core set of map-assigned co-dominant, single locus SSR markers has been defined for whole genome scans of genetic variability in white clover.
Original languageEnglish
Pages (from-to)531-539
Number of pages9
JournalPlant Science
Issue number3
Early online date11 Jun 2003
Publication statusPublished - 01 Sept 2003


  • Molecular marker
  • Simple sequence repeats
  • Trifolium repens
  • Genetic map
  • Linkage group
  • DNA sequence analysis


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