Antigen specificity in experimental bovine tuberculosis

S. G. Rhodes*, D. Gavier-Widen, B. M. Buddle, A. O. Whelan, M. Singh, R. G. Hewinson, H. M. Vordermeier

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

51 Citations (SciVal)

Abstract

This report describes the kinetics of T-cell responses to a panel of mycobacterial antigens (PPD-M, PPD-A, ESAT-6, Ag85, 38kD, MPB64, MPB70, MPB83, hsp 16.1, hsp65, and hsp70) following experimental infection of cattle with Mycobacterium bovis. Increased antigen-specific lymphocyte proliferation, gamma interferon, and interleukin-2 responses were observed in all calves following infection. Positive lymphocyte proliferation and cytokine responses to PPD-M and ESAT-6 were observed throughout the infection period studied. In contrast, responses to all other antigens were more variable and were not constantly present, suggesting that antigen cocktails rather than individual antigens should be used for immunodiagnosis. The detection of cytokine responses in the absence of lymphocyte proliferation, particularly during the early stages of infection, suggests a role for antigen-specific cytokine readout systems in the early identification of M. bovis infection in cattle.

Original languageEnglish
Pages (from-to)2573-2578
Number of pages6
JournalInfection and Immunity
Volume68
Issue number5
DOIs
Publication statusPublished - 01 May 2000

Keywords

  • Animals
  • Antigens, Bacterial/immunology
  • Cattle
  • Cell Division
  • Female
  • Interferon-gamma/biosynthesis
  • Interleukin-2/biosynthesis
  • Leukocytes, Mononuclear/immunology
  • Mycobacterium bovis/immunology
  • Tuberculosis, Bovine/immunology

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