TY - JOUR
T1 - Application of flow cytometry for the determination of minimal inhibitory concentration of several antibacterial agents on Mycoplasma hyopneumoniae
AU - Assunção, Patrícia
AU - Antunes, Nuno T.
AU - Rosales, R. S.
AU - Poveda, Carlos
AU - de la Fe, Christian
AU - Poveda, José B.
AU - Davey, Hazel M.
N1 - P. Assunção, N.T. Antunes, R.S. Rosales, C. Poveda, C. de la Fe, J.B. Poveda and H.M. Davey (2007). Application of flow cytometry for the determination of minimal inhibitory concentration of several antibacterial agents on Mycoplasma hyopneumoniae. Journal of Applied Microbiology, 102(4), 1132-1137.
Sponsorship: Gobierno de Canarias (IDT-LP-04/016)
RAE2008
PY - 2007/4/1
Y1 - 2007/4/1
N2 - Aim: In this study, flow cytometry was evaluated for the determination of the minimal inhibitory concentrations (MICs) of nine antibacterial agents (enrofloxacin, ciprofloxacin, oxytetracycline, chloramphenicol, tylosin, lincomycin, gentamycin, spectinomycin and streptomycin) against M. hyopneumoniae.
Methods and Results: Flow cytometry was able to detect Mycoplasma hyopneumoniae inhibition at 12 h postincubation, whereas the results obtained by the traditional method were only obtained at 48 h, when a visible change in the medium had occurred. At 48 h, both methods gave the same result for eight antibacterial agents, whereas flow cytometry gave slightly higher MIC values for one antibacterial agent (tylosin). This was attributed to the fact that the M. hyopneumoniae growth that had occurred in those tubes was not enough to visibly change the colour of the medium. A good relationship was found between the flow cytometry and the traditional method.
Conclusion: Flow cytometry was found to be a good method for the determination of antimicrobial MICs in M. hyopneumoniae.
Significance and Impact of the Study: The flow cytometric method allows the determination of the response of M. hyopneumoniae to each of the antibacterial agents in near real time, and has potential for the identification and study of resistant subpopulations.
AB - Aim: In this study, flow cytometry was evaluated for the determination of the minimal inhibitory concentrations (MICs) of nine antibacterial agents (enrofloxacin, ciprofloxacin, oxytetracycline, chloramphenicol, tylosin, lincomycin, gentamycin, spectinomycin and streptomycin) against M. hyopneumoniae.
Methods and Results: Flow cytometry was able to detect Mycoplasma hyopneumoniae inhibition at 12 h postincubation, whereas the results obtained by the traditional method were only obtained at 48 h, when a visible change in the medium had occurred. At 48 h, both methods gave the same result for eight antibacterial agents, whereas flow cytometry gave slightly higher MIC values for one antibacterial agent (tylosin). This was attributed to the fact that the M. hyopneumoniae growth that had occurred in those tubes was not enough to visibly change the colour of the medium. A good relationship was found between the flow cytometry and the traditional method.
Conclusion: Flow cytometry was found to be a good method for the determination of antimicrobial MICs in M. hyopneumoniae.
Significance and Impact of the Study: The flow cytometric method allows the determination of the response of M. hyopneumoniae to each of the antibacterial agents in near real time, and has potential for the identification and study of resistant subpopulations.
KW - antibiotics
KW - flow cytometry
KW - minimal inhibitory concentrations
KW - Mycoplasma hyopneumoniae
KW - SUSCEPTIBILITY
U2 - 10.1111/j.1365-2672.2006.03170.x
DO - 10.1111/j.1365-2672.2006.03170.x
M3 - Article
SN - 1364-5072
VL - 102
SP - 1132
EP - 1137
JO - Journal of Applied Microbiology
JF - Journal of Applied Microbiology
IS - 4
ER -