TY - JOUR
T1 - Application of GISH and AFLP techniques for identification of Lolium-Festuca introgressions
AU - Skibinska, Maria
AU - Kosmala, A.
AU - Humphreys, Michael W.
AU - Zwierzykowski, Z.
N1 - Skibinska, M., Kosmala, A., Humphreys, M. W., Zwierzykowski, Z. (2002). Application of GISH and AFLP techniques for identification of Lolium-Festuca introgressions. Cellular & Molecular Biology Letters, 7, (2A), 493-498.
PY - 2002
Y1 - 2002
N2 - At present, breeding programmes aimed at combining advantageous traits within the Lolium-Festuca complex, are mainly focused on introgression procedures. One principal objective, is the transfer of genes conferring resistance to abiotic stresses from Festuca species (F. pratensis, F. arundinacea and F. glaucescens) into Lolium multiflorum and L. perenne germplasm. In our experiments, two different hybrids: triploid – L. multiflorum (4x) × F. pratensis (2x) and pentaploid – F. arundinacea (6x) × L. multiflorum (4x) were backcrossed twice onto L. multiflorum cultivars, and numerous BC2 progeny generated. BC2 plants from both combinations were tested in field and/or simulated conditions for winter hardiness and drought resistance. GISH (genomic in situ hybridisation) analyses were then performed on the most winter hardy and drought resistant plants to locate putative genes for stress resistance. Using resistant L. multiflorum genotypes with a single Festuca chromatin segment, it was possible to allocate AFLP (amplified fragment length polymorphism) markers specific to that segment. Markers associated with genes conferring stress resistance facilitate marker-assisted selection programmes to obtain new, more persistent grass cultivars. Preliminary results of GISH analysis, to identify Festuca chromosome segments in L. multiflorum introgression lines and to find segment-specific AFLP markers, are presented.
AB - At present, breeding programmes aimed at combining advantageous traits within the Lolium-Festuca complex, are mainly focused on introgression procedures. One principal objective, is the transfer of genes conferring resistance to abiotic stresses from Festuca species (F. pratensis, F. arundinacea and F. glaucescens) into Lolium multiflorum and L. perenne germplasm. In our experiments, two different hybrids: triploid – L. multiflorum (4x) × F. pratensis (2x) and pentaploid – F. arundinacea (6x) × L. multiflorum (4x) were backcrossed twice onto L. multiflorum cultivars, and numerous BC2 progeny generated. BC2 plants from both combinations were tested in field and/or simulated conditions for winter hardiness and drought resistance. GISH (genomic in situ hybridisation) analyses were then performed on the most winter hardy and drought resistant plants to locate putative genes for stress resistance. Using resistant L. multiflorum genotypes with a single Festuca chromatin segment, it was possible to allocate AFLP (amplified fragment length polymorphism) markers specific to that segment. Markers associated with genes conferring stress resistance facilitate marker-assisted selection programmes to obtain new, more persistent grass cultivars. Preliminary results of GISH analysis, to identify Festuca chromosome segments in L. multiflorum introgression lines and to find segment-specific AFLP markers, are presented.
M3 - Article
SN - 1425-8153
VL - 7
SP - 493
EP - 498
JO - Cellular and Molecular Biology Letters
JF - Cellular and Molecular Biology Letters
IS - 2A
ER -