AQUA: automated quality improvement for multiple sequence alignments

Jean Muller, Christopher J. Creevey, Julie D. Thompson, Detlev Arendt, Peer Bork

Research output: Contribution to journalArticlepeer-review

45 Citations (SciVal)


Multiple sequence alignment (MSA) is a central tool in most modern biology studies. However, despite generations of valuable tools, human experts are still able to improve automatically generated MSAs. In an effort to automatically identify the most reliable MSA for a given protein family, we propose a very simple protocol, named AQUA for 'Automated quality improvement for multiple sequence alignments'. Our current implementation relies on two alignment programs (MUSCLE and MAFFT), one refinement program (RASCAL) and one assessment program (NORMD), but other programs could be incorporated at any of the three steps. Availability: AQUA is implemented in Tcl/Tk and runs in command line on all platforms. The source code is available under the GNU GPL license. Source code, README and Supplementary data are available at
Original languageEnglish
Pages (from-to)263-265
Number of pages3
Issue number2
Early online date19 Nov 2009
Publication statusPublished - 2010


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