Assessment of cross-reactivity between Mycobacterium bovis and M. kansasii ESAT-6 and CFP-10 at the T-cell epitope level

H. Martin Vordermeier*, Jemma Brown, Paul J. Cockle, Willeke P.J. Franken, Sandra M. Arend, Tom H.M. Ottenhoff, Keith Jahans, R. Glyn Hewinson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

47 Citations (SciVal)

Abstract

Cross-reactivity between Mycobacterium kansasii ESAT-6 and CFP-10 homologues and their M. bovis counterparts can confound the interpretation of immunodiagnostic tests for tuberculosis. M. kansasii is a nontuberculous mycobacterial species cultured from skin test-positive cattle in Great Britain. Using peptides derived from M. bovis and M. kansasii ESAT-6 and CFP-10 regions that differ between these species, we investigated the species specificity and cross-reactivity at the level of individual bovine T-cell epitopes. Our results demonstrated that all peptides tested are fully cross-reactive, with the exception of one ESAT-6-derived peptide that harbored an M. bovis-specific epitope(s) when it was recognized in the context of bovine leukocyte antigen (BoLA)-DQ but that was cross-reactive with its M. kansasii homologues when it was restricted by BoLA-DR. This observation further highlights that prediction of species specificity by comparing sequence identity/homology alone is not sufficient and that individuals with diverse major histocompatibility complex constellations need to be tested to characterize the cross-reactivity or species specificity of peptide-based reagents.

Original languageEnglish
Pages (from-to)1203-1209
Number of pages7
JournalClinical and Vaccine Immunology
Volume14
Issue number9
Early online date01 Aug 2007
DOIs
Publication statusPublished - 01 Sept 2007

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