Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks

Javier Bezos, Julio Álvarez, Lucía de Juan, Beatriz Romero, Sabrina Rodríguez, Isabel G. Fernández-de-Mera, R. Glyn Hewinson, Martin Vordermeier, Ana Mateos, Lucas Domínguez, Alicia Aranaz*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (SciVal)


Caprine tuberculosis in Spain is mainly caused by Mycobacterium caprae although the progression of the disease and lesion severity is similar to that caused by Mycobacterium bovis. In this study, the sensitivity of the gamma-interferon (IFN-γ) assay using an antigen cocktail containing early secretory antigenic target-6. kDa (ESAT-6) and culture filtrate protein 10 (CFP-10) peptides for stimulation was determined and compared with those obtained in single intradermal tuberculin (SIT) and single intradermal cervical comparative tuberculin (SICCT) tests and IFN-γ assay using purified protein derivative (PPD) in three different flocks infected with M. caprae under different epidemiological conditions. Correlation between specific IFN-γ production and severity of lesions was also evaluated. Sensitivities of the diagnostic tests varied greatly in the three flocks studied, with higher values in those where higher lesion scores were observed. The results show that IFN-γ assay applied in goats using PPD or the ESAT-6/CFP-10 peptides cocktail for stimulation yielded similar sensitivity values. A significant yet weak positive correlation between specific IFN-γ production and lesion scores was detected after the stimulation with PPDs (p= 0.004) whereas when the blood samples were stimulated with ESAT-6/CFP-10 peptides, the correlation was not significant (p> 0.05). Therefore, specific-IFN-γ production after the stimulation with PPDs or ESAT-6/CFP-10 was not an accurate indicator of lesion severity in naturally tuberculosis infected goats with M. caprae.

Original languageEnglish
Pages (from-to)187-192
Number of pages6
JournalPreventive Veterinary Medicine
Issue number3-4
Early online date04 May 2011
Publication statusPublished - 01 Jul 2011


  • Diagnosis
  • Goat
  • IFN-γ assay
  • Lesion score
  • Sensitivity
  • Tuberculosis


Dive into the research topics of 'Assessment of in vivo and in vitro tuberculosis diagnostic tests in Mycobacterium caprae naturally infected caprine flocks'. Together they form a unique fingerprint.

Cite this