Baseline Gut Microbiota Composition Is Associated With Schistosoma mansoni Infection Burden in Rodent Models

Alba Cortés, Simon Clare, Alice Costain, Alexandre Almeida, Catherine McCarthy, Katherine Harcourt, Cordelia Brandt, Charlotte Tolley, James Rooney, Matthew Berriman, Trevor Lawley, Andrew S. MacDonald, Gabriel Rinaldi, Cinzia Cantacessi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (SciVal)

Abstract

In spite of growing evidence supporting the occurrence of complex interactions between Schistosoma and gut bacteria in mice and humans, no data is yet available on whether worm-mediated changes in microbiota composition are dependent on the baseline gut microbial profile of the vertebrate host. In addition, the impact of such changes on the susceptibility to, and pathophysiology of, schistosomiasis remains largely unexplored. In this study, mice colonized with gut microbial populations from a human donor (HMA mice), as well as microbiota-wild type (WT) animals, were infected with Schistosoma mansoni, and alterations of their gut microbial profiles at 50 days post-infection were compared to those occurring in uninfected HMA and WT rodents, respectively. Significantly higher worm and egg burdens, together with increased specific antibody responses to parasite antigens, were observed in HMA compared to WT mice. These differences were associated to extensive dissimilarities between the gut microbial profiles of each HMA and WT groups of mice at baseline; in particular, the gut microbiota of HMA animals was characterized by low microbial alpha diversity and expanded Proteobacteria, as well as by the absence of putative immunomodulatory bacteria (e.g. Lactobacillus). Furthermore, differences in infection-associated changes in gut microbiota composition were observed between HMA and WT mice. Altogether, our findings support the hypothesis that susceptibility to S. mansoni infection in mice is partially dependent on the composition of the host baseline microbiota. Moreover, this study highlights the applicability of HMA mouse models to address key biological questions on host-parasite-microbiota relationships in human helminthiases.

Original languageEnglish
Article number593838
JournalFrontiers in Immunology
Volume11
DOIs
Publication statusPublished - 18 Nov 2020
Externally publishedYes

Keywords

  • dysbiosis
  • gut microbial diversity
  • helminth-gut microbiota interactions
  • human-microbiota associated mouse models
  • immune-modulation
  • Schistosoma mansoni
  • Bacteria/classification
  • Immunomodulation
  • Parasite Load
  • Schistosoma
  • Biodiversity
  • Computational Biology/methods
  • Dysbiosis
  • Host-Parasite Interactions/immunology
  • Metagenomics/methods
  • Animals
  • Feces/microbiology
  • Gastrointestinal Microbiome/immunology
  • Antibodies, Protozoan/immunology
  • Mice
  • RNA, Ribosomal, 16S
  • Schistosomiasis mansoni/immunology
  • Disease Models, Animal

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