Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta

Peter M. Brophy, John Barrett, N. Saghir, Phillip J. Conde

Research output: Contribution to journalArticlepeer-review

21 Citations (SciVal)

Abstract

The cestode Hymenolepis diminuta contains an abundant, cytoplasmic, hydrophobic ligand, binding protein (H-HLBP). Studies with polarity sensitive probes suggest a single hydrophobic binding site, the results also indicate that the single tryptophan in the molecule (Trp41) is involved in ligand binding. Of the possible physiological ligands tested, only haematin and retinoids (retinol and retinoic acid) show appreciable binding in addition to fatty acids. H-HLBP also binds a range of anthelmintics, again with KD values in the nM range. The interaction of anthelmintics with hydrophobic binding proteins may be important in determining drug specificity and site of action and could have a role in the development of drug resistance.
Original languageEnglish
Pages (from-to)653-660
Number of pages8
JournalInternational Journal for Parasitology
Volume31
Issue number7
DOIs
Publication statusPublished - 15 May 2001

Keywords

  • Hydrophobic ligand binding protein
  • Hymenolepis diminuta; Cestoda
  • 11-[5-dimethylaminonaphthalene-1-sulphonyl amino] undecanoic acid (DAUDA)
  • Anthelmintic

Fingerprint

Dive into the research topics of 'Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta'. Together they form a unique fingerprint.

Cite this