TY - JOUR
T1 - Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta
AU - Brophy, Peter M.
AU - Barrett, John
AU - Saghir, N.
AU - Conde, Phillip J.
N1 - Saghir, N., Conde, P. J., Brophy, P. M., Barrett, J. (2001). Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta. International Journal for Parasitology, 31, (7), 653-660
Sponsorship: Wellcome Trust (047546); BBSRC
PY - 2001/5/15
Y1 - 2001/5/15
N2 - The cestode Hymenolepis diminuta contains an abundant, cytoplasmic, hydrophobic ligand, binding protein (H-HLBP). Studies with polarity sensitive probes suggest a single hydrophobic binding site, the results also indicate that the single tryptophan in the molecule (Trp41) is involved in ligand binding. Of the possible physiological ligands tested, only haematin and retinoids (retinol and retinoic acid) show appreciable binding in addition to fatty acids. H-HLBP also binds a range of anthelmintics, again with KD values in the nM range. The interaction of anthelmintics with hydrophobic binding proteins may be important in determining drug specificity and site of action and could have a role in the development of drug resistance.
AB - The cestode Hymenolepis diminuta contains an abundant, cytoplasmic, hydrophobic ligand, binding protein (H-HLBP). Studies with polarity sensitive probes suggest a single hydrophobic binding site, the results also indicate that the single tryptophan in the molecule (Trp41) is involved in ligand binding. Of the possible physiological ligands tested, only haematin and retinoids (retinol and retinoic acid) show appreciable binding in addition to fatty acids. H-HLBP also binds a range of anthelmintics, again with KD values in the nM range. The interaction of anthelmintics with hydrophobic binding proteins may be important in determining drug specificity and site of action and could have a role in the development of drug resistance.
KW - Hydrophobic ligand binding protein
KW - Hymenolepis diminuta; Cestoda
KW - 11-[5-dimethylaminonaphthalene-1-sulphonyl amino] undecanoic acid (DAUDA)
KW - Anthelmintic
U2 - 10.1016/S0020-7519(01)00135-7
DO - 10.1016/S0020-7519(01)00135-7
M3 - Article
SN - 0020-7519
VL - 31
SP - 653
EP - 660
JO - International Journal for Parasitology
JF - International Journal for Parasitology
IS - 7
ER -