Biolistics transformation of wheat

Caroline A. Sparks, Huw D Jones

Research output: Chapter in Book/Report/Conference proceedingChapter

41 Citations (SciVal)

Abstract

We present a complete, step-by-step guide to the production of transformed wheat plants using a particle bombardment device to deliver plasmid DNA into immature embryos and the regeneration of transgenic plants via somatic embryogenesis. Currently, this is the most commonly used method for transforming wheat and it offers some advantages. However, it will be interesting to see whether this position is challenged as facile methods are developed for delivering DNA by Agrobacterium tumefaciens or by the production of transformants via a germ-line process (see other chapters in this book).

Original languageEnglish
Title of host publicationTransgenic Wheat, Barley and Oats
Subtitle of host publicationProduction and Characterization Protocols
EditorsHuw D. Jones, Peter R. Shewry
PublisherSpringer Nature
Pages71-92
Number of pages22
Volume478
ISBN (Electronic)978-1-59745-379-0
ISBN (Print)978-1-58829-961-1
DOIs
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
Volume478
ISSN (Print)1064-3745

Keywords

  • Biolistics
  • DNA, Plant
  • Gold
  • Plants, Genetically Modified
  • Triticum
  • wheat
  • particle bombardment
  • transgenic plant

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