Biolistics transformation of wheat

Caroline A. Sparks, Huw D Jones

Research output: Chapter in Book/Report/Conference proceedingChapter

41 Citations (SciVal)


We present a complete, step-by-step guide to the production of transformed wheat plants using a particle bombardment device to deliver plasmid DNA into immature embryos and the regeneration of transgenic plants via somatic embryogenesis. Currently, this is the most commonly used method for transforming wheat and it offers some advantages. However, it will be interesting to see whether this position is challenged as facile methods are developed for delivering DNA by Agrobacterium tumefaciens or by the production of transformants via a germ-line process (see other chapters in this book).

Original languageEnglish
Title of host publicationTransgenic Wheat, Barley and Oats
Subtitle of host publicationProduction and Characterization Protocols
EditorsHuw D. Jones, Peter R. Shewry
PublisherSpringer Nature
Number of pages22
ISBN (Electronic)978-1-59745-379-0
ISBN (Print)978-1-58829-961-1
Publication statusPublished - 2009

Publication series

NameMethods in Molecular Biology
PublisherHumana Press
ISSN (Print)1064-3745


  • Biolistics
  • DNA, Plant
  • Gold
  • Plants, Genetically Modified
  • Triticum
  • wheat
  • particle bombardment
  • transgenic plant


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