Biomarkers of cell-mediated immunity to bovine tuberculosis

Mitchell V. Palmer*, Tyler C. Thacker, Meaghan M. Rabideau, Gareth J. Jones, Carly Kanipe, H. Martin Vordermeier, W. Ray Waters

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (SciVal)

Abstract

Whole blood based assays, particularly interferon gamma (IFN-γ) release assays (IGRAs), are used for the diagnosis of both bovine and human tuberculosis (TB). The aim of the current study was to evaluate a panel of cytokines and chemokines for potential use as diagnostic readouts indicative of Mycobacterium bovis (M. bovis) infection in cattle. A gene expression assay was used to determine the kinetics of the response to M. bovis purified protein derivative and a fusion protein consisting of ESAT-6, CFP10, and Rv3615c upon aerosol infection with ∼104 cfu of M. bovis. The panel of biomarkers included: IFN-γ, CXCL9, CXCL10, CCL2, CCL3, TNF-α, IL-1α, IL-1β, IL-1Ra, IL-22, IL-21 and IL-13. Protein levels of IFN-γ, CXCL9, and CXCL10 were determined by ELISA. Findings suggest that CXCL9, CXCL10, IL-21, IL-13, and several acute phase cytokines may be worth pursuing as diagnostic biomarkers of M. bovis infection in cattle.

Original languageEnglish
Article number109988
JournalVeterinary Immunology and Immunopathology
Volume220
Early online date30 Nov 2019
DOIs
Publication statusPublished - 01 Feb 2020

Keywords

  • Chemokines
  • Cytokines
  • Mycobacterium bovis
  • Tuberculosis
  • Whole blood rtPCR
  • Tuberculosis, Bovine/blood
  • Interferon-gamma
  • Gene Expression
  • Chemokine CXCL9/blood
  • Interferon-gamma Release Tests
  • Male
  • Chemokine CXCL10/blood
  • Animals
  • Cattle
  • Cattle Diseases/blood
  • Cytokines/genetics
  • Biomarkers/blood
  • Immunity, Cellular

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