TY - JOUR
T1 - Biomarkers of cell-mediated immunity to bovine tuberculosis
AU - Palmer, Mitchell V.
AU - Thacker, Tyler C.
AU - Rabideau, Meaghan M.
AU - Jones, Gareth J.
AU - Kanipe, Carly
AU - Vordermeier, H. Martin
AU - Ray Waters, W.
N1 - Funding Information:
This work was supported by the US Department of Agriculture, Agricultural Research Service Project (CRIS# 5030-32000-222) and the Department for Environment, Food and Rural Affairs (DEFRA), UK (HMV). USDA is an equal opportunity employer.
Funding Information:
This work was supported by the US Department of Agriculture, Agricultural Research Service Project (CRIS# 5030-32000-222 ) and the Department for Environment, Food and Rural Affairs (DEFRA), UK (HMV). USDA is an equal opportunity employer. Appendix A
Publisher Copyright:
© 2019
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Whole blood based assays, particularly interferon gamma (IFN-γ) release assays (IGRAs), are used for the diagnosis of both bovine and human tuberculosis (TB). The aim of the current study was to evaluate a panel of cytokines and chemokines for potential use as diagnostic readouts indicative of Mycobacterium bovis (M. bovis) infection in cattle. A gene expression assay was used to determine the kinetics of the response to M. bovis purified protein derivative and a fusion protein consisting of ESAT-6, CFP10, and Rv3615c upon aerosol infection with ∼104 cfu of M. bovis. The panel of biomarkers included: IFN-γ, CXCL9, CXCL10, CCL2, CCL3, TNF-α, IL-1α, IL-1β, IL-1Ra, IL-22, IL-21 and IL-13. Protein levels of IFN-γ, CXCL9, and CXCL10 were determined by ELISA. Findings suggest that CXCL9, CXCL10, IL-21, IL-13, and several acute phase cytokines may be worth pursuing as diagnostic biomarkers of M. bovis infection in cattle.
AB - Whole blood based assays, particularly interferon gamma (IFN-γ) release assays (IGRAs), are used for the diagnosis of both bovine and human tuberculosis (TB). The aim of the current study was to evaluate a panel of cytokines and chemokines for potential use as diagnostic readouts indicative of Mycobacterium bovis (M. bovis) infection in cattle. A gene expression assay was used to determine the kinetics of the response to M. bovis purified protein derivative and a fusion protein consisting of ESAT-6, CFP10, and Rv3615c upon aerosol infection with ∼104 cfu of M. bovis. The panel of biomarkers included: IFN-γ, CXCL9, CXCL10, CCL2, CCL3, TNF-α, IL-1α, IL-1β, IL-1Ra, IL-22, IL-21 and IL-13. Protein levels of IFN-γ, CXCL9, and CXCL10 were determined by ELISA. Findings suggest that CXCL9, CXCL10, IL-21, IL-13, and several acute phase cytokines may be worth pursuing as diagnostic biomarkers of M. bovis infection in cattle.
KW - Chemokines
KW - Cytokines
KW - Mycobacterium bovis
KW - Tuberculosis
KW - Whole blood rtPCR
KW - Tuberculosis, Bovine/blood
KW - Interferon-gamma
KW - Gene Expression
KW - Chemokine CXCL9/blood
KW - Interferon-gamma Release Tests
KW - Male
KW - Chemokine CXCL10/blood
KW - Animals
KW - Cattle
KW - Cattle Diseases/blood
KW - Cytokines/genetics
KW - Biomarkers/blood
KW - Immunity, Cellular
UR - http://www.scopus.com/inward/record.url?scp=85076271629&partnerID=8YFLogxK
U2 - 10.1016/j.vetimm.2019.109988
DO - 10.1016/j.vetimm.2019.109988
M3 - Article
C2 - 31846797
AN - SCOPUS:85076271629
SN - 0165-2427
VL - 220
JO - Veterinary Immunology and Immunopathology
JF - Veterinary Immunology and Immunopathology
M1 - 109988
ER -
