Botrytis cinerea Loss and Restoration of Virulence during In Vitro Culture Follows Flux in Global DNA Methylation

James Breen, Luis Alejandro Jose Mur, Anushen Sivakumaran, Aderemi Akinyemi, Michael James Wilkinson, Carlos Marcelino Rodriguez Lopez*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (SciVal)
29 Downloads (Pure)

Abstract

Pathogenic fungi can lose virulence after protracted periods of culture, but little is known of the underlying mechanisms. Here, we present the first analysis of DNA methylation flux at a single-base resolution for the plant pathogen B. cinerea and identify differentially methylated genes/genomic regions associated with virulence erosion during in vitro culture. Cultures were maintained for eight months, with subcultures and virulence testing every month. Methylationsensitive amplified polymorphisms were performed at monthly intervals to characterise global changes to the pathogen’s genome during culture and also on DNA from mycelium inoculated onto Arabidopsis thaliana after eight months in culture. Characterisation of culture-induced epialleles was assessed by whole-genome re-sequencing and whole-genome bisulfite sequencing. Virulence declined with time in culture and recovered after inoculation on A. thaliana. Variation detected by methylation-sensitive amplified polymorphisms followed virulence changes during culture. Wholegenome (bisulfite) sequencing showed marked changes in global and local methylation during culture but no significant genetic changes. We imply that virulence is a non-essential plastic character that is at least partly modified by the changing levels of DNA methylation during culture. We hypothesise that changing DNA methylation during culture may be responsible for the high virulence/low virulence transition in B. cinerea and speculate that this may offer fresh opportunities to control pathogen virulence.

Original languageEnglish
Article number3034
Number of pages19
JournalInternational Journal of Molecular Sciences
Volume23
Issue number6
DOIs
Publication statusPublished - 11 Mar 2022

Keywords

  • Epigenetic
  • Fungal pathogen culture
  • Grey mould fungus
  • Methylation Sensitive Amplified Polymorphisims
  • Virulent
  • Whole Genome Bisulfite Sequencing
  • Botrytis/genetics
  • DNA Methylation
  • Arabidopsis/genetics
  • Host-Pathogen Interactions/genetics
  • Virulence/genetics

Fingerprint

Dive into the research topics of 'Botrytis cinerea Loss and Restoration of Virulence during In Vitro Culture Follows Flux in Global DNA Methylation'. Together they form a unique fingerprint.

Cite this