Carcinogenic liver fluke Opisthorchis viverrini oxysterols detected by LC-MS/MS survey of soluble fraction parasite extract

Nuno Vale*, Maria João Gouveia, Mónica Botelho, Banchob Sripa, Sutas Suttiprapa, Gabriel Rinaldi, Paula Gomes, Paul J. Brindley, José Manuel Correia da Costa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Citations (SciVal)

Abstract

Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone.

Original languageEnglish
Pages (from-to)535-542
Number of pages8
JournalParasitology International
Volume62
Issue number6
Early online date20 Aug 2013
DOIs
Publication statusPublished - 01 Dec 2013
Externally publishedYes

Keywords

  • Amino Acid Sequence
  • Animals
  • Bile Acids and Salts/metabolism
  • Cholesterol/analogs & derivatives
  • Chromatography, Liquid/veterinary
  • Cricetinae
  • Cyprinidae
  • Fish Diseases/parasitology
  • Helminth Proteins/metabolism
  • Humans
  • Metacercariae
  • Opisthorchiasis/parasitology
  • Opisthorchis/chemistry
  • Oxidation-Reduction
  • Tandem Mass Spectrometry/veterinary

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