CDKG1 protein kinase is essential for synapsis and male meiosis at high ambient temperature in Arabidopsis thaliana

Tao Zheng, Candida Nibau, Dylan W. Phillips, Glyn Jenkins, Susan J. Armstrong, John H. Doonan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)
184 Downloads (Pure)

Abstract

The Arabidopsis cyclin-dependent kinase G (CDKG) gene defines a clade of cyclin-dependent protein kinases related to CDK10 and CDK11, as well as to the enigmatic Ph1-related kinases that are implicated in controlling homeologous chromosome pairing in wheat. Here we demonstrate that the CDKG1/CYCLINL complex is essential for synapsis and recombination during male meiosis. A transfer-DNA insertional mutation in the cdkg1 gene leads to a temperature-sensitive failure of meiosis in late Zygotene/Pachytene that is associated with defective formation of the synaptonemal complex, reduced bivalent formation and crossing over, and aneuploid gametes. An aphenotypic insertion in the cyclin L gene, a cognate cyclin for CDKG, strongly enhances the phenotype of cdkg1-1 mutants, indicating that this cdk-cyclin complex is essential for male meiosis. Since CYCLINL, CDKG, and their mammalian homologs have been previously shown to affect mRNA processing, particularly alternative splicing, our observations also suggest a mechanism to explain the widespread phenomenon of thermal sensitivity in male meiosis.

Original languageEnglish
Pages (from-to)2182-2187
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number6
DOIs
Publication statusPublished - 11 Feb 2014

Keywords

  • TRITICUM-AESTIVUM
  • PROPHASE-I
  • MEIOTIC RECOMBINATION
  • SYNAPTONEMAL COMPLEX
  • CHROMOSOME SYNAPSIS
  • CHIASMA FORMATION
  • PH1 LOCUS
  • WHEAT
  • MUTANTS
  • PROGRESSION
  • Arabidopsis Proteins/physiology
  • Hot Temperature
  • Chromosomes, Plant
  • Chromosome Pairing/physiology
  • Protein Kinases/metabolism
  • Polymerase Chain Reaction
  • Arabidopsis/enzymology
  • Pollen

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