Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment

Qiang Li; Andrew J.E. Bettany; Iain Donnison; Catherine M. Griffiths; Howard Thomas; Ian M. Scott

doi:10.1016/S0167-4781(00)00077-4

Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment

Qiang Li, Andrew J.E. Bettany^*, Iain Donnison, Catherine M. Griffiths, Howard Thomas, Ian M. Scott

^*Corresponding author for this work

Prifysgol Aberystwyth | Aberystwyth University

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

A cysteine protease cDNA clone (See1) highly homologous to barley aleurain was isolated from Lolium multiflorum leaves. During leaf senescence, expression of the See1 mRNA and protein was strongly enhanced. In dark-incubated leaf segments, cytokinin delayed senescence and reduced expression of both See1 mRNA and protein.

Original language	English
Pages (from-to)	233-236
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1492
Issue number	1
DOIs	https://doi.org/10.1016/S0167-4781(00)00077-4
Publication status	Published - 21 Jun 2000

Keywords

Cysteine protease
Cytokinin
Leaf senescence
Lolium multiflorum
Ryegrass
Gene Expression/drug effects
Amino Acid Sequence
Molecular Sequence Data
DNA, Plant/analysis
RNA, Messenger/metabolism
Sequence Homology, Amino Acid
Cellular Senescence/genetics
DNA, Complementary/analysis
Cytokinins/pharmacology
Plant Leaves/drug effects
Lolium/drug effects
Cysteine Endopeptidases/biosynthesis

Access to Document

10.1016/S0167-4781(00)00077-4

Permanent link

Persistent link

Cite this

@article{3a537949477044529be7e18cb3813642,

title = "Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment",

abstract = "A cysteine protease cDNA clone (See1) highly homologous to barley aleurain was isolated from Lolium multiflorum leaves. During leaf senescence, expression of the See1 mRNA and protein was strongly enhanced. In dark-incubated leaf segments, cytokinin delayed senescence and reduced expression of both See1 mRNA and protein.",

keywords = "Cysteine protease, Cytokinin, Leaf senescence, Lolium multiflorum, Ryegrass, Gene Expression/drug effects, Amino Acid Sequence, Molecular Sequence Data, DNA, Plant/analysis, RNA, Messenger/metabolism, Sequence Homology, Amino Acid, Cellular Senescence/genetics, DNA, Complementary/analysis, Cytokinins/pharmacology, Plant Leaves/drug effects, Lolium/drug effects, Cysteine Endopeptidases/biosynthesis",

author = "Qiang Li and Bettany, {Andrew J.E.} and Iain Donnison and Griffiths, {Catherine M.} and Howard Thomas and Scott, {Ian M.}",

year = "2000",

month = jun,

day = "21",

doi = "10.1016/S0167-4781(00)00077-4",

language = "English",

volume = "1492",

pages = "233--236",

journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",

issn = "0167-4781",

publisher = "Elsevier",

number = "1",

}

Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment. / Li, Qiang; Bettany, Andrew J.E.; Donnison, Iain et al.
In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1492, No. 1, 21.06.2000, p. 233-236.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment

AU - Li, Qiang

AU - Bettany, Andrew J.E.

AU - Donnison, Iain

AU - Griffiths, Catherine M.

AU - Thomas, Howard

AU - Scott, Ian M.

PY - 2000/6/21

Y1 - 2000/6/21

N2 - A cysteine protease cDNA clone (See1) highly homologous to barley aleurain was isolated from Lolium multiflorum leaves. During leaf senescence, expression of the See1 mRNA and protein was strongly enhanced. In dark-incubated leaf segments, cytokinin delayed senescence and reduced expression of both See1 mRNA and protein.

AB - A cysteine protease cDNA clone (See1) highly homologous to barley aleurain was isolated from Lolium multiflorum leaves. During leaf senescence, expression of the See1 mRNA and protein was strongly enhanced. In dark-incubated leaf segments, cytokinin delayed senescence and reduced expression of both See1 mRNA and protein.

KW - Cysteine protease

KW - Cytokinin

KW - Leaf senescence

KW - Lolium multiflorum

KW - Ryegrass

KW - Gene Expression/drug effects

KW - Amino Acid Sequence

KW - Molecular Sequence Data

KW - DNA, Plant/analysis

KW - RNA, Messenger/metabolism

KW - Sequence Homology, Amino Acid

KW - Cellular Senescence/genetics

KW - DNA, Complementary/analysis

KW - Cytokinins/pharmacology

KW - Plant Leaves/drug effects

KW - Lolium/drug effects

KW - Cysteine Endopeptidases/biosynthesis

UR - http://www.scopus.com/inward/record.url?scp=0034697729&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(00)00077-4

DO - 10.1016/S0167-4781(00)00077-4

M3 - Article

C2 - 11004495

AN - SCOPUS:0034697729

SN - 0167-4781

VL - 1492

SP - 233

EP - 236

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

Characterisation of a cysteine protease cDNA from Lolium multiflorum leaves and its expression during senescence and cytokinin treatment

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this