Abstract
We have previously shown that the secreted M. tuberculosis complex proteins CFP-10 and ESAT-6 form a tight, 1:1 complex, which may represent their functional form. In the work reported here a combination of yeast two-hybrid and biochemical analysis has been used to characterise complex formation between two other pairs of CFP-10/ESAT-6 family proteins (Rv0287/Rv0288 and Rv3019c/Rv3020c) and to determine whether complexes can be formed between non-genome paired members of the family. The results clearly demonstrate that Rv0287/Rv0288 and Rv3019c/3020c form tight complexes, as initially observed for CFP-10/ESAT-6. The closely related Rv0287/Rv0288 and Rv3019c/Rv3020c proteins are also able to form non-genome paired complexes (Rv0287/Rv3019c and Rv0288/Rv3020c), but are not capable of binding to the more distantly related CFP-10/ESAT-6 proteins.
Original language | English |
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Pages (from-to) | 255-262 |
Number of pages | 8 |
Journal | FEMS Microbiology Letters |
Volume | 238 |
Issue number | 1 |
Early online date | 29 Jul 2004 |
DOIs | |
Publication status | Published - 01 Sept 2004 |
Keywords
- CFP-10
- ESAT-6
- ESAT-6 family members
- Protein-protein interaction
- Tuberculosis