TY - JOUR
T1 - Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand
AU - Holder, Angela
AU - Kolakowski, Jeannine
AU - Rosentreter, Chloe
AU - Knuepfer, Ellen
AU - Jégouzo, Sabine A. F.
AU - Rosenwasser, Oliver
AU - Harris, Heather
AU - Baumgaertel, Lotta
AU - Gibson, Amanda
AU - Werling, Dirk
N1 - Funding Information:
This work was supported by BBSRC grants BB/P005659/1 and BB/P008461/1. Acknowledgments
Publisher Copyright:
Copyright © 2023 Holder, Kolakowski, Rosentreter, Knuepfer, Jégouzo, Rosenwasser, Harris, Baumgaertel, Gibson and Werling.
PY - 2023/5/19
Y1 - 2023/5/19
N2 - Innate immune receptors that form complexes with secondary receptors, activating multiple signalling pathways, modulate cellular activation and play essential roles in regulating homeostasis and immunity. We have previously identified a variety of bovine C-type lectin-like receptors that possess similar functionality than their human orthologues. Mincle (CLEC4E), a heavily glycosylated monomer, is involved in the recognition of the mycobacterial component Cord factor (trehalose 6,6′-dimycolate). Here we characterise the bovine homologue of Mincle (boMincle), and demonstrate that the receptor is structurally and functionally similar to the human orthologue (huMincle), although there are some notable differences. In the absence of cross-reacting antibodies, boMincle-specific antibodies were created and used to demonstrate that, like the human receptor, boMincle is predominantly expressed by myeloid cells. BoMincle surface expression increases during the maturation of monocytes to macrophages. However, boMincle mRNA transcripts were also detected in granulocytes, B cells, and T cells. Finally, we show that boMincle binds to isolated bovine CD4+ T cells in a specific manner, indicating the potential to recognise endogenous ligands. This suggests that the receptor might also play a role in homeostasis in cattle.
AB - Innate immune receptors that form complexes with secondary receptors, activating multiple signalling pathways, modulate cellular activation and play essential roles in regulating homeostasis and immunity. We have previously identified a variety of bovine C-type lectin-like receptors that possess similar functionality than their human orthologues. Mincle (CLEC4E), a heavily glycosylated monomer, is involved in the recognition of the mycobacterial component Cord factor (trehalose 6,6′-dimycolate). Here we characterise the bovine homologue of Mincle (boMincle), and demonstrate that the receptor is structurally and functionally similar to the human orthologue (huMincle), although there are some notable differences. In the absence of cross-reacting antibodies, boMincle-specific antibodies were created and used to demonstrate that, like the human receptor, boMincle is predominantly expressed by myeloid cells. BoMincle surface expression increases during the maturation of monocytes to macrophages. However, boMincle mRNA transcripts were also detected in granulocytes, B cells, and T cells. Finally, we show that boMincle binds to isolated bovine CD4+ T cells in a specific manner, indicating the potential to recognise endogenous ligands. This suggests that the receptor might also play a role in homeostasis in cattle.
KW - innate immunity
KW - C-type lectin receptors
KW - bovine Mincle
KW - pattern recognition receptors
KW - Cord Factors
KW - Animals
KW - Signal Transduction
KW - Cattle
KW - Humans
KW - Receptors, Immunologic/metabolism
KW - Ligands
KW - Lectins, C-Type/metabolism
UR - http://www.scopus.com/inward/record.url?scp=85160974125&partnerID=8YFLogxK
U2 - 10.3389/fimmu.2023.1189587
DO - 10.3389/fimmu.2023.1189587
M3 - Article
C2 - 37275870
SN - 1664-3224
VL - 14
JO - Frontiers in Immunology
JF - Frontiers in Immunology
M1 - 1189587
ER -