Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand

Angela Holder, Jeannine Kolakowski, Chloe Rosentreter, Ellen Knuepfer, Sabine A. F. Jégouzo, Oliver Rosenwasser, Heather Harris, Lotta Baumgaertel, Amanda Gibson, Dirk Werling*

*Corresponding author for this work

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Innate immune receptors that form complexes with secondary receptors, activating multiple signalling pathways, modulate cellular activation and play essential roles in regulating homeostasis and immunity. We have previously identified a variety of bovine C-type lectin-like receptors that possess similar functionality than their human orthologues. Mincle (CLEC4E), a heavily glycosylated monomer, is involved in the recognition of the mycobacterial component Cord factor (trehalose 6,6′-dimycolate). Here we characterise the bovine homologue of Mincle (boMincle), and demonstrate that the receptor is structurally and functionally similar to the human orthologue (huMincle), although there are some notable differences. In the absence of cross-reacting antibodies, boMincle-specific antibodies were created and used to demonstrate that, like the human receptor, boMincle is predominantly expressed by myeloid cells. BoMincle surface expression increases during the maturation of monocytes to macrophages. However, boMincle mRNA transcripts were also detected in granulocytes, B cells, and T cells. Finally, we show that boMincle binds to isolated bovine CD4+ T cells in a specific manner, indicating the potential to recognise endogenous ligands. This suggests that the receptor might also play a role in homeostasis in cattle.
Original languageEnglish
Article number1189587
Number of pages14
JournalFrontiers in Immunology
Publication statusPublished - 19 May 2023


  • innate immunity
  • C-type lectin receptors
  • bovine Mincle
  • pattern recognition receptors
  • Cord Factors
  • Animals
  • Signal Transduction
  • Cattle
  • Humans
  • Receptors, Immunologic/metabolism
  • Ligands
  • Lectins, C-Type/metabolism


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