Characterization of newly established bovine intestinal epithelial cell line

Kohtaro Miyazawa, Tetsuya Hondo, Takashi Kanaya, Sachi Tanaka, Ikuro Takakura, Wataru Itani, Michael Terence Rose, Haruki Kitazawa, Takahiro Yamaguchi, Hisashi Aso

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56 Citations (SciVal)


Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer's patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells.
Original languageEnglish
Pages (from-to)125-134
Number of pages10
JournalHistochemistry and Cell Biology
Issue number1
Publication statusPublished - Jan 2010


  • Bovine small intestine
  • Epithelial cell line
  • Cell differentiation
  • M cells
  • Peyer’s patches


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