TY - JOUR
T1 - Chromatographic separation of mixed peptides from amino acids in biological digests with volatile buffers
AU - Armstead, Ian
AU - Ling, John
N1 - Funding Information:
The authors wish to acknowledge the financial support of the Agricultural and Food Research Council, UK.
PY - 1991/11/22
Y1 - 1991/11/22
N2 - Two chromatographic methods, capable of separating mixed peptides from contaminating amino acids in biological digests, are described. Both methods involve separation on copper-Chelex resin, but each uses a different set of elution buffers. When separation method 1 was applied to a commercially available proteolytic digest of casein, the free amino acid content was reduced from 26.0% to 0.5%. With an enzymic digest of 14C-labelled proteins derived from plant biomass, separation method 2 decreased the contaminating free amino acids from 20.3% to 1.9%. Since the separated peptides are eluted with volatile buffers, they are suitable as substrates for biological experiments
AB - Two chromatographic methods, capable of separating mixed peptides from contaminating amino acids in biological digests, are described. Both methods involve separation on copper-Chelex resin, but each uses a different set of elution buffers. When separation method 1 was applied to a commercially available proteolytic digest of casein, the free amino acid content was reduced from 26.0% to 0.5%. With an enzymic digest of 14C-labelled proteins derived from plant biomass, separation method 2 decreased the contaminating free amino acids from 20.3% to 1.9%. Since the separated peptides are eluted with volatile buffers, they are suitable as substrates for biological experiments
UR - http://www.scopus.com/inward/record.url?scp=0025996886&partnerID=8YFLogxK
U2 - 10.1016/0021-9673(91)85130-8
DO - 10.1016/0021-9673(91)85130-8
M3 - Article
SN - 0021-9673
VL - 586
SP - 259
EP - 263
JO - Journal of Chromatography A
JF - Journal of Chromatography A
IS - 2
ER -