Chromosome pairing in Lolium perenne x L. temulentum diploid hybrids: Genetic cytogenetic evaluation

Ian Armstead; A. Bollard; I. P. King; J. W. Forster; M. D. Hayward; G. M. Evans; H. M. Thomas

doi:10.1038/sj.hdy.6885730

Chromosome pairing in Lolium perenne x L. temulentum diploid hybrids: Genetic cytogenetic evaluation

Ian Armstead, A. Bollard, I. P. King, J. W. Forster, M. D. Hayward, G. M. Evans, H. M. Thomas

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

A Lolium perenne genotype (E5/2/5/10), which had been selected for low chiasma frequency over a number of generations and which was suspected of containing one or two heterozygous dominant genes with a significant effect on chiasma frequency, was crossed with L. temulentum (Ba3081) to create a hybrid population of 47 diploid plants. The mean chiasma or paired arm (PA) frequency of homoeologous chromosomes at meiosis in the population was 9.1/cell (1.3 PA/chromosome pair) with a distribution skewed towards high PA frequency. More than 90% of the hybrid chromosomes paired at meiosis in spite of the disparity in chromosome length and DNA quantity between the two species. Overall, the distribution of PAs between chromosomes for a given number of PAs/cell favoured the production of rod bivalents over ring bivalents and univalents, indicating that there is a mechanism present that maximizes the total number of bivalent associations formed. Molecular marker analysis using AFLPs and isoenzymes did not identify any clear major gene effect on PA frequency in the hybrid population. It was concluded that the control of PA frequency in E5/2/5/10 was not a simple genetic mechanism.

Original language	English
Pages (from-to)	298-303
Number of pages	6
Journal	Heredity
Volume	83
Issue number	3
DOIs	https://doi.org/10.1038/sj.hdy.6885730
Publication status	Published - 01 Sept 1999

Keywords

Chiasma-frequency
Cytogenetics
Genetics
Lolium

Access to Document

10.1038/sj.hdy.6885730

Permanent link

Persistent link

Cite this

@article{6336499eb7894325960396e5ec9594a2,

title = "Chromosome pairing in Lolium perenne x L. temulentum diploid hybrids: Genetic cytogenetic evaluation",

abstract = "A Lolium perenne genotype (E5/2/5/10), which had been selected for low chiasma frequency over a number of generations and which was suspected of containing one or two heterozygous dominant genes with a significant effect on chiasma frequency, was crossed with L. temulentum (Ba3081) to create a hybrid population of 47 diploid plants. The mean chiasma or paired arm (PA) frequency of homoeologous chromosomes at meiosis in the population was 9.1/cell (1.3 PA/chromosome pair) with a distribution skewed towards high PA frequency. More than 90% of the hybrid chromosomes paired at meiosis in spite of the disparity in chromosome length and DNA quantity between the two species. Overall, the distribution of PAs between chromosomes for a given number of PAs/cell favoured the production of rod bivalents over ring bivalents and univalents, indicating that there is a mechanism present that maximizes the total number of bivalent associations formed. Molecular marker analysis using AFLPs and isoenzymes did not identify any clear major gene effect on PA frequency in the hybrid population. It was concluded that the control of PA frequency in E5/2/5/10 was not a simple genetic mechanism.",

keywords = "Chiasma-frequency, Cytogenetics, Genetics, Lolium",

author = "Ian Armstead and A. Bollard and King, {I. P.} and Forster, {J. W.} and Hayward, {M. D.} and Evans, {G. M.} and Thomas, {H. M.}",

note = "Funding Information: The authors would like to thank Mr C. Evans (IGER), Dr M. O. Humphreys (IGER) and Mr D. Fallding (University Wales, Aberystwyth) for their expertise in isoenzyme analysis, statistical analysis and embryo rescue, respectively. This research was supported by the BBSRC through the Plant and Animal Genome Analysis Programme (PAG/04444).",

year = "1999",

month = sep,

day = "1",

doi = "10.1038/sj.hdy.6885730",

language = "English",

volume = "83",

pages = "298--303",

journal = "Heredity",

issn = "0018-067X",

publisher = "Springer Nature",

number = "3",

}

TY - JOUR

T1 - Chromosome pairing in Lolium perenne x L. temulentum diploid hybrids

T2 - Genetic cytogenetic evaluation

AU - Armstead, Ian

AU - Bollard, A.

AU - King, I. P.

AU - Forster, J. W.

AU - Hayward, M. D.

AU - Evans, G. M.

AU - Thomas, H. M.

N1 - Funding Information: The authors would like to thank Mr C. Evans (IGER), Dr M. O. Humphreys (IGER) and Mr D. Fallding (University Wales, Aberystwyth) for their expertise in isoenzyme analysis, statistical analysis and embryo rescue, respectively. This research was supported by the BBSRC through the Plant and Animal Genome Analysis Programme (PAG/04444).

PY - 1999/9/1

Y1 - 1999/9/1

N2 - A Lolium perenne genotype (E5/2/5/10), which had been selected for low chiasma frequency over a number of generations and which was suspected of containing one or two heterozygous dominant genes with a significant effect on chiasma frequency, was crossed with L. temulentum (Ba3081) to create a hybrid population of 47 diploid plants. The mean chiasma or paired arm (PA) frequency of homoeologous chromosomes at meiosis in the population was 9.1/cell (1.3 PA/chromosome pair) with a distribution skewed towards high PA frequency. More than 90% of the hybrid chromosomes paired at meiosis in spite of the disparity in chromosome length and DNA quantity between the two species. Overall, the distribution of PAs between chromosomes for a given number of PAs/cell favoured the production of rod bivalents over ring bivalents and univalents, indicating that there is a mechanism present that maximizes the total number of bivalent associations formed. Molecular marker analysis using AFLPs and isoenzymes did not identify any clear major gene effect on PA frequency in the hybrid population. It was concluded that the control of PA frequency in E5/2/5/10 was not a simple genetic mechanism.

AB - A Lolium perenne genotype (E5/2/5/10), which had been selected for low chiasma frequency over a number of generations and which was suspected of containing one or two heterozygous dominant genes with a significant effect on chiasma frequency, was crossed with L. temulentum (Ba3081) to create a hybrid population of 47 diploid plants. The mean chiasma or paired arm (PA) frequency of homoeologous chromosomes at meiosis in the population was 9.1/cell (1.3 PA/chromosome pair) with a distribution skewed towards high PA frequency. More than 90% of the hybrid chromosomes paired at meiosis in spite of the disparity in chromosome length and DNA quantity between the two species. Overall, the distribution of PAs between chromosomes for a given number of PAs/cell favoured the production of rod bivalents over ring bivalents and univalents, indicating that there is a mechanism present that maximizes the total number of bivalent associations formed. Molecular marker analysis using AFLPs and isoenzymes did not identify any clear major gene effect on PA frequency in the hybrid population. It was concluded that the control of PA frequency in E5/2/5/10 was not a simple genetic mechanism.

KW - Chiasma-frequency

KW - Cytogenetics

KW - Genetics

KW - Lolium

UR - http://www.scopus.com/inward/record.url?scp=0033197531&partnerID=8YFLogxK

U2 - 10.1038/sj.hdy.6885730

DO - 10.1038/sj.hdy.6885730

M3 - Article

SN - 0018-067X

VL - 83

SP - 298

EP - 303

JO - Heredity

JF - Heredity

IS - 3

ER -

Chromosome pairing in Lolium perenne x L. temulentum diploid hybrids: Genetic cytogenetic evaluation

Abstract

Keywords

Access to Document

Permanent link

Other files and links

Fingerprint

Cite this