Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro

G. Gyulai, Mervyn O. Humphreys, A. Bittsanszky, Kirsten P. Skøt, J. Kiss, Leif Skøt, G. Gullner, Susan Heywood, Z. Szabo, J. Alan Lovatt, L. Radimszky, Hywel W. Roderick, Michael T. Abberton, R. Lagler, H. Rennenberg, T. Komives, L. E. Heszky

Research output: Chapter in Book/Report/Conference proceedingConference Proceeding (Non-Journal item)


In vitro phytoextraction ability of vegetative clones of Populus canescens including two transgenic clones (ggs11 and lg16) was studied in leaf disc cultures. Clone stability was determined by fAFLP (fluorescent labelled amplified fragment length polymorphism). In total, 679 AFLP fragments were identified. Only one of them was different (99.85% genetic similarity). Presence of the gsh1 transgene in the transformed clones (ggs11 and lg16) was detected in PCR reactions using gsh1 specific primers. For the study of phytoextraction activity, leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10-1 to 10-5 M) incubated on tissue culture media WPM for 21 days. Zn2+ showed phytotoxicity only at high concentrations (10-1 to 10-2 M). Transgenic poplars showed elevated heavy metal (Zn and Cu) uptake compared to the non-transformed clones. These results suggest that transgenic poplars may be suitable for phytoremediation of soils contaminated with Zn2+
Original languageEnglish
Title of host publicationGenetic Variation for Plant Breeding
EditorsJ. Vollmann, H. Grausgruber, P. Ruckenbauer
PublisherUniversity of Natural Resources and Life Sciences, Vienna
Number of pages5
ISBN (Print)3900962561
Publication statusPublished - 2004
EventProceedings of the 17th Eucarpial General Congress - Tulln, Austria
Duration: 08 Sept 200411 Sept 2004


ConferenceProceedings of the 17th Eucarpial General Congress
Period08 Sept 200411 Sept 2004


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