Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro

G. Gyulai; Mervyn O. Humphreys; A. Bittsanszky; Kirsten P. Skøt; J. Kiss; Leif Skøt; G. Gullner; Susan Heywood; Z. Szabo; J. Alan Lovatt; L. Radimszky; Hywel W. Roderick; Michael T. Abberton; R. Lagler; H. Rennenberg; T.  Komives; L. E. Heszky

Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro

G. Gyulai, Mervyn O. Humphreys, A. Bittsanszky, Kirsten P. Skøt, J. Kiss, Leif Skøt, G. Gullner, Susan Heywood, Z. Szabo, J. Alan Lovatt, L. Radimszky, Hywel W. Roderick, Michael T. Abberton, R. Lagler, H. Rennenberg, T. Komives, L. E. Heszky

Department of Life Sciences

Research output: Chapter in Book/Report/Conference proceeding › Conference Proceeding (Non-Journal item)

Abstract

In vitro phytoextraction ability of vegetative clones of Populus canescens including two transgenic clones (ggs11 and lg16) was studied in leaf disc cultures. Clone stability was determined by fAFLP (fluorescent labelled amplified fragment length polymorphism). In total, 679 AFLP fragments were identified. Only one of them was different (99.85% genetic similarity). Presence of the gsh1 transgene in the transformed clones (ggs11 and lg16) was detected in PCR reactions using gsh1 specific primers. For the study of phytoextraction activity, leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10-1 to 10-5 M) incubated on tissue culture media WPM for 21 days. Zn2+ showed phytotoxicity only at high concentrations (10-1 to 10-2 M). Transgenic poplars showed elevated heavy metal (Zn and Cu) uptake compared to the non-transformed clones. These results suggest that transgenic poplars may be suitable for phytoremediation of soils contaminated with Zn2+

Original language	English
Title of host publication	Genetic Variation for Plant Breeding
Editors	J. Vollmann, H. Grausgruber, P. Ruckenbauer
Publisher	University of Natural Resources and Life Sciences, Vienna
Pages	279-283
Number of pages	5
ISBN (Print)	3900962561
Publication status	Published - 2004
Event	Proceedings of the 17th Eucarpial General Congress - Tulln, Austria Duration: 08 Sept 2004 → 11 Sept 2004

Conference

Conference	Proceedings of the 17th Eucarpial General Congress
Country/Territory	Austria
City	Tulln
Period	08 Sept 2004 → 11 Sept 2004

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Gyulai, G., Humphreys, M. O., Bittsanszky, A., Skøt, K. P., Kiss, J., Skøt, L., Gullner, G., Heywood, S., Szabo, Z., Lovatt, J. A., Radimszky, L., Roderick, H. W., Abberton, M. T., Lagler, R., Rennenberg, H., Komives, T., & Heszky, L. E. (2004). Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro. In J. Vollmann, H. Grausgruber, & P. Ruckenbauer (Eds.), Genetic Variation for Plant Breeding (pp. 279-283). University of Natural Resources and Life Sciences, Vienna. http://hdl.handle.net/2160/3157

@inproceedings{e100465f2975432f958ec6006387298e,

title = "Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro",

abstract = "In vitro phytoextraction ability of vegetative clones of Populus canescens including two transgenic clones (ggs11 and lg16) was studied in leaf disc cultures. Clone stability was determined by fAFLP (fluorescent labelled amplified fragment length polymorphism). In total, 679 AFLP fragments were identified. Only one of them was different (99.85% genetic similarity). Presence of the gsh1 transgene in the transformed clones (ggs11 and lg16) was detected in PCR reactions using gsh1 specific primers. For the study of phytoextraction activity, leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10-1 to 10-5 M) incubated on tissue culture media WPM for 21 days. Zn2+ showed phytotoxicity only at high concentrations (10-1 to 10-2 M). Transgenic poplars showed elevated heavy metal (Zn and Cu) uptake compared to the non-transformed clones. These results suggest that transgenic poplars may be suitable for phytoremediation of soils contaminated with Zn2+",

author = "G. Gyulai and Humphreys, {Mervyn O.} and A. Bittsanszky and Sk{\o}t, {Kirsten P.} and J. Kiss and Leif Sk{\o}t and G. Gullner and Susan Heywood and Z. Szabo and Lovatt, {J. Alan} and L. Radimszky and Roderick, {Hywel W.} and Abberton, {Michael T.} and R. Lagler and H. Rennenberg and T. Komives and Heszky, {L. E.}",

note = "Gyulai, G., Humphreys, M. O., Bittsanszky, A., Sk{\o}t, K. P., Kiss, J., Sk{\o}t, L., Gullner, G., Heywood, S., Szabo, Z., Lovatt, J. A., Radimszky, L., Roderick, H. W., Abberton, M. T., Lagler, R., Rennenberg, H., Komives, T., Heszky, L. E. (2004). Clonal propagation and improved phytoextraction activity of gshl poplar clones (Populus canescens) in vitro. Pages 279-283 in: Vollmann, J., Grausgruber, H., Ruckenbauer, P. (Eds). Genetic Variation for Plant Breeding. Proceedings of the 17th Eucarpia General Congress, Tulln, Austria, 8-11 September 2004.; Proceedings of the 17th Eucarpial General Congress ; Conference date: 08-09-2004 Through 11-09-2004",

year = "2004",

language = "English",

isbn = "3900962561",

pages = "279--283",

editor = "J. Vollmann and H. Grausgruber and P. Ruckenbauer",

booktitle = "Genetic Variation for Plant Breeding",

publisher = "University of Natural Resources and Life Sciences, Vienna",

address = "Austria",

}

Gyulai, G, Humphreys, MO, Bittsanszky, A, Skøt, KP, Kiss, J, Skøt, L, Gullner, G, Heywood, S, Szabo, Z, Lovatt, JA, Radimszky, L, Roderick, HW, Abberton, MT, Lagler, R, Rennenberg, H, Komives, T & Heszky, LE 2004, Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro. in J Vollmann, H Grausgruber & P Ruckenbauer (eds), Genetic Variation for Plant Breeding. University of Natural Resources and Life Sciences, Vienna, pp. 279-283, Proceedings of the 17th Eucarpial General Congress, Tulln, Austria, 08 Sept 2004. <http://hdl.handle.net/2160/3157>

Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro. / Gyulai, G.; Humphreys, Mervyn O.; Bittsanszky, A. et al.
Genetic Variation for Plant Breeding. ed. / J. Vollmann; H. Grausgruber; P. Ruckenbauer. University of Natural Resources and Life Sciences, Vienna, 2004. p. 279-283.

Research output: Chapter in Book/Report/Conference proceeding › Conference Proceeding (Non-Journal item)

TY - GEN

T1 - Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro

AU - Gyulai, G.

AU - Humphreys, Mervyn O.

AU - Bittsanszky, A.

AU - Skøt, Kirsten P.

AU - Kiss, J.

AU - Skøt, Leif

AU - Gullner, G.

AU - Heywood, Susan

AU - Szabo, Z.

AU - Lovatt, J. Alan

AU - Radimszky, L.

AU - Roderick, Hywel W.

AU - Abberton, Michael T.

AU - Lagler, R.

AU - Rennenberg, H.

AU - Komives, T.

AU - Heszky, L. E.

N1 - Gyulai, G., Humphreys, M. O., Bittsanszky, A., Skøt, K. P., Kiss, J., Skøt, L., Gullner, G., Heywood, S., Szabo, Z., Lovatt, J. A., Radimszky, L., Roderick, H. W., Abberton, M. T., Lagler, R., Rennenberg, H., Komives, T., Heszky, L. E. (2004). Clonal propagation and improved phytoextraction activity of gshl poplar clones (Populus canescens) in vitro. Pages 279-283 in: Vollmann, J., Grausgruber, H., Ruckenbauer, P. (Eds). Genetic Variation for Plant Breeding. Proceedings of the 17th Eucarpia General Congress, Tulln, Austria, 8-11 September 2004.

PY - 2004

Y1 - 2004

N2 - In vitro phytoextraction ability of vegetative clones of Populus canescens including two transgenic clones (ggs11 and lg16) was studied in leaf disc cultures. Clone stability was determined by fAFLP (fluorescent labelled amplified fragment length polymorphism). In total, 679 AFLP fragments were identified. Only one of them was different (99.85% genetic similarity). Presence of the gsh1 transgene in the transformed clones (ggs11 and lg16) was detected in PCR reactions using gsh1 specific primers. For the study of phytoextraction activity, leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10-1 to 10-5 M) incubated on tissue culture media WPM for 21 days. Zn2+ showed phytotoxicity only at high concentrations (10-1 to 10-2 M). Transgenic poplars showed elevated heavy metal (Zn and Cu) uptake compared to the non-transformed clones. These results suggest that transgenic poplars may be suitable for phytoremediation of soils contaminated with Zn2+

AB - In vitro phytoextraction ability of vegetative clones of Populus canescens including two transgenic clones (ggs11 and lg16) was studied in leaf disc cultures. Clone stability was determined by fAFLP (fluorescent labelled amplified fragment length polymorphism). In total, 679 AFLP fragments were identified. Only one of them was different (99.85% genetic similarity). Presence of the gsh1 transgene in the transformed clones (ggs11 and lg16) was detected in PCR reactions using gsh1 specific primers. For the study of phytoextraction activity, leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10-1 to 10-5 M) incubated on tissue culture media WPM for 21 days. Zn2+ showed phytotoxicity only at high concentrations (10-1 to 10-2 M). Transgenic poplars showed elevated heavy metal (Zn and Cu) uptake compared to the non-transformed clones. These results suggest that transgenic poplars may be suitable for phytoremediation of soils contaminated with Zn2+

M3 - Conference Proceeding (Non-Journal item)

SN - 3900962561

SP - 279

EP - 283

BT - Genetic Variation for Plant Breeding

A2 - Vollmann, J.

A2 - Grausgruber, H.

A2 - Ruckenbauer, P.

PB - University of Natural Resources and Life Sciences, Vienna

T2 - Proceedings of the 17th Eucarpial General Congress

Y2 - 8 September 2004 through 11 September 2004

ER -

Clonal propagation and improved phytoextraction activity of gshI poplar clones (Populus canescens ) in vitro

Abstract

Conference

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